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TranscriptomicsmRNA cap methylation in pluripotency and differentiation
The mRNA cap recruits factors essential for transcript processing and translation initiation. We report that regulated mRNA cap methylation is a feature of embryonic stem cell (ESC) differentiation. Expression of the mRNA cap methyltransferase activating subunit, RAM, is elevated in ESCs resulting i...
ORGANISM(S): Mus musculus 
2016-06-14 | E-GEOD-81849 | biostudies-arrayexpress
TranscriptomicsCharacterization of human malaria parasite Plasmodium falciparum eIF4E homologue and mRNA 5′ cap status
The mRNA 5′ cap is normally essential for eukaryotic mRNA translation, stabilization and transport and both the cap and eIF4E are important elements of post-transcriptional gene regulation. To further our understanding of mRNA translation in the human malaria parasite Plasmodium falciparum, we hav...
ORGANISM(S): Plasmodium falciparum 
2010-06-10 | E-GEOD-6356 | biostudies-arrayexpress
Transcriptomicsm6A in 5’ untranslated regions promotes cap-independent translation of mRNA
N6-methyladenosine (m6A) is a widespread internal RNA modification whose function is poorly understood. Here we report that m6A residues within the 5'UTR promote a novel form of cap-independent translation which is mediated through an interaction between m6A residues and the translation initiation f...
ORGANISM(S): Homo sapiens 
2015-10-26 | E-GEOD-73405 | biostudies-arrayexpress
TranscriptomicsSequencing the cap-snatching repertoire of H1N1 influenza provides insight into the mechanism of viral transcription initiation
The influenza polymerase cleaves host RNAs ~10–13 nucleotides downstream of their 5′ ends and uses this capped fragment to prime viral mRNA synthesis. To better understand this process of cap snatching, we used high-throughput sequencing to determine the 5′ ends of A/WSN/33 (H1N1) influenza mRNAs. T...
ORGANISM(S): Influenza A virus (A/WSN/1933(H1N1)) 
2015-04-23 | E-GEOD-65101 | biostudies-arrayexpress
ProteomicsDAP5 directs a widespread alternate form of cap-dependent mRNA translation initiation using eIF3d for specialized cell functions
Affinity purification of eIF4GII, eIF4GI and DAP5 using a HA tag on the N-terminus of each protein.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-05-25 | MSV000082407 | MassIVE
TranscriptomicsmiRNA expression profiles in the 19 candidate urines (prostate cancer, CaP, patients and non-cancer subjects with benign prostatic hyperplasia, BPH)
The aims of the study were investigated whether expression of urinary cell-free miRNAs would be different between prostate cancer (CaP) patients and non-cancer subjects with benign prostatic hyperplasia (BPH). Urinary cell-free miRNAs will provide potential benefits for detecting CaP, and virus-enco...
ORGANISM(S): Homo sapiens 
2014-01-14 | E-GEOD-54010 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of the radicle and micropylar endosperm cap of Lepidium seeds during germination in control medium
An experiment was performed to compare global transcription patterns in three tissues of Lepidium sativum seeds at different times during imbibition leading up to, but not including, radicle protrusion from the seed (e.g. germination). RNA extractions from the three tissues were hybridised to CATMA ...
ORGANISM(S): Lepidium sativum 
2010-06-23 | E-TABM-745 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of the radicle and micropylar endosperm cap of Lepidium seeds during germination in ABA
An experiment was performed to compare global transcription patterns in two tissues of Lepidium sativum seeds at different times during imbibition leading up to, but not including, radicle protrusion from the seed (e.g. germination). RNA extractions from the two tissues were hybridised to CATMA Arab...
ORGANISM(S): Lepidium sativum 
2010-10-02 | E-TABM-743 | biostudies-arrayexpress
MetabolomicsLipid metabolites as potential diagnostic and prognostic biomarkers for acute community acquired pneumonia
Early diagnosis of acute community-acquired pneumonia (CAP) is important in patient triage and treatment decisions. To identify biomarkers that distinguish patients with CAP from non-CAP controls, we conducted an untargeted global metabolome analysis for plasma samples from142 patients with CAP (CAP...
2016-08-11 | MTBLS354 | MetaboLights
TranscriptomicsDevelopment of GRO-cap (GRO-seq followed by 5'-cap enrichment) to map transcription start sites in C. elegans
Dosage Compensation is required to correct for uneven gene dose between the sexes. We utilized global run-on sequencing (GRO-seq) to examine how Caenorhabditis elegans dosage compensation reduces transcription of X-linked genes. To facilitate these experiments, we required accurate 5M-bM-^@M-^Y-ends...
ORGANISM(S): Caenorhabditis elegans 
2013-06-18 | E-GEOD-43085 | biostudies-arrayexpress
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