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Secretory Carrier-Associated Membrane Proteins (SCAMPs) are highly conserved proteins that are involved in membrane trafficking. An integrated approach was taken to elucidate function of SCAMPs in wood formation of Populus trees. The metabolomics analyses were performed in woody tissues of transgeni...
2018-01-08 | MTBLS478 | MetaboLights
A role of chromatin in plant memory of environmental stress has been proposed. In this study ChIP-Seq was employed to investigate whether a transient mild salt treatment of Arabidopsis thaliana plant at seedling stage (M-^Qpriming treatmentM-^R: 50 mM NaCl for 24 hours) altered genome-wide profiles ...
ORGANISM(S): Arabidopsis thaliana 
E. coli MG1655 was grown on M9 minimal medium supplemented with 15mM glucose and either 0, 10, 50 or 100 mM acetate. Transcriptomic analysis from exponential growing cells was performed using Agilent microarray A-MEXP-2232. This work was done with the help of students during practicals. Consequentl...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
We have established Drosophila melanogaster as a model system for ocular hypertension by expressing wild-type human myocilin (MYOC) in the Drosophila eye. Here, we have created transgenic flies that express four clinically relevant mutant forms of MYOC (R342K, Q368X, D380N and K423E) in their eyes ...
ORGANISM(S): Drosophila melanogaster 
As the application of carbon nanotubes (CNT) in consumer products continues to rise, studies have expanded to determine the associated risks of exposure on human and environmental health. In particular, several lines of evidence indicate that exposure to multi-walled carbon nanotubes (MWCNT) could p...
ORGANISM(S): Homo sapiens 
We used microarrays to assess whole genome transcript profiles of the 40 homozygous Raleigh lines to understand the genetic basis of complex traits in Drosophila
ORGANISM(S): Drosophila melanogaster 
The poly(A) tail at 3' ends of eukaryotic mRNAs promotes their nuclear export, stability and translational efficiency, and changes in its length can strongly impact gene expression. The Arabidopsis thaliana genome encodes three canonical nuclear poly(A) polymerases, PAPS1, PAPS2 and PAPS4. As shown ...
ORGANISM(S): Arabidopsis thaliana 
A relationship between transcript level and histone modifications is apparent in steady state but their dynamic relationship remains to be understood. In this study we used RNA-sequencing to determine transcript levels in Arabidopsis thaliana plants after a short, mild salt treatment at seedling ...
ORGANISM(S): Arabidopsis thaliana 
Epistasis and pleiotropy are important features of the genetic architecture of quantitative traits, but are difficult to assess in outbred populations. We performed a diallel cross among Drosophila P-element mutations previously associated with hyper-aggressive behavior, and demonstrated extensive e...
ORGANISM(S): Drosophila melanogaster 
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