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TranscriptomicsSequencing of reaction products from protospacer integration by Cas1-Cas2
We report the sites of protospacer integration into plasmids by Cas1-Cas2. The goal of this study is to determine the role of sequence elements on protospacer integration. Methods: Cas1-Cas2 integration products were fragmented, end-repaired, adapter ligated, PCR amplified, and analyzed by Illumina ...
ORGANISM(S): Cloning vector pUC19 
2015-01-30 | E-GEOD-64552 | biostudies-arrayexpress
ProteomicsCas1-Cas2 and DnaK physically and functionally interact to modulate CRISPR Adaptation
Prokaryotic Cas1-Cas2 protein complexes generate adaptive immunity to mobile genetic elements (MGEs), by capture and integration of MGE DNA in to CRISPR sites. De novo immunity relies on Cas1-Cas2 targeting MGE DNA, without the aid of pre-existing immunity complexes, through mechanisms of ‘naive ada...
ORGANISM(S): Escherichia coli 
2023-05-18 | PXD042090 | Pride
Crispr Cas1-cas2 Adaptaion Dnak 
OtherProtospacer integration into plasmid DNA by Cas1–Cas2
We report the integration site specificity of Cas1–Cas2 into target plasmid DNA encoding a CRISPR locus. The goal of this study is to determine the effect of IHF on target site selection of Cas1–Cas2.
ORGANISM(S): Cloning vector pUC19 
2016-05-27 | GSE79992 | GEO
OtherSequencing of reaction products from protospacer integration by Cas1-Cas2
We report the sites of protospacer integration into plasmids by Cas1-Cas2. The goal of this study is to determine the role of sequence elements on protospacer integration.
ORGANISM(S): Cloning vector pUC19 
2015-01-30 | GSE64552 | GEO
UnknownAllosteric regulation in CRISPR/Cas1-Cas2 protospacer acquisition mediated by DNA and Cas2.
Not available
S-EPMC8390960 | biostudies-literature
UnknownCas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity.
Not available
S-EPMC4075942 | biostudies-literature
UnknownThe Cas4-Cas1-Cas2 complex mediates precise prespacer processing during CRISPR adaptation.
Not available
S-EPMC6519985 | biostudies-literature
UnknownPAM binding ensures orientational integration during Cas4-Cas1-Cas2-mediated CRISPR adaptation.
Not available
S-EPMC9675710 | biostudies-literature
UnknownCas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity.
Not available
S-EPMC5495223 | biostudies-literature
UnknownSpacer capture and integration by a type I-F Cas1-Cas2-3 CRISPR adaptation complex.
Not available
S-EPMC5495228 | biostudies-literature
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