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TranscriptomicsMeasuring transcriptional differences between Caulobacter crescentus cultured in nitrate versus ammonium as the sole nitrogen source
This study is measuring the steady-state levels of mRNA in wild-type Caulobacter crescentus grown in M2 defined medium containing either ammonium or nitrate as the sole nitrogen source. Four independent cultures of Caulobacter crecentus were grown in each of two medium conditions: M2(nitrate)glucose...
ORGANISM(S): Caulobacter vibrioides 
2010-06-20 | E-GEOD-21203 | biostudies-arrayexpress
TranscriptomicsThe coding and noncoding architecture of the Caulobacter crescentus genome
Caulobacter crescentus undergoes an asymmetric cell division controlled by a genetic circuit that cycles in space and time. We provide a universal strategy for defining the coding potential of bacterial genomes by applying ribosome profiling, RNA-seq, global 5’-RACE, and liquid chromatography coupl...
ORGANISM(S): Caulobacter crescentus NA1000 
2014-05-29 | E-GEOD-54883 | biostudies-arrayexpress
TranscriptomicsTesting the effects of nitric oxide (oxidative) stress on Caulobacter transcription
The goal of this study was to measure the effects of nitric oxide exposure (using DETA NONOate as a nitric oxide donor) on transcription in Caulobacter. Untreated Caulobacter crescentus were grown to a density of 0.3 (at OD660) in PYE medium (pH 7) in rolled culture tubes. DETA-NONOate treated Cau...
ORGANISM(S): Caulobacter vibrioides 
2010-06-20 | E-GEOD-21139 | biostudies-arrayexpress
GenomicsIdentification of the PhoB regulon and role of PhoU in the phosphate-starvation response of Caulobacter crescentus (ChIP-seq)
An ability to sense and respond to changes in extracellular phosphate is critical to the survival of most bacteria. For Caulobacter crescentus, which typically lives in phosphate-limited environments, this process is especially crucial. Like many bacteria, Caulobacter responds to phosphate limitatio...
ORGANISM(S): Caulobacter crescentus NA1000 
2016-08-06 | E-GEOD-71860 | biostudies-arrayexpress
ProteomicsCaulobacter crescentus flagellins glycopeptides analyses
Caulobacter crescentus is an alphaproteobacterium that divides assymetrically. Each cell cycle results in the production of a motile flagellated cell and a sessile cell called the swamer cell and the stalked cell, respectively. The flagellar filament is composed of thousands polymerized flagellins. ...
ORGANISM(S): Brevundimonas subvibrioides ATCC 15264 Caulobacter crescentus (strain NA1000 / CB15N) 
2023-01-13 | PXD038730 | Pride
Caulobacter crescentus Glycopeptides Flagellins 
TranscriptomicsHigh-resolution mapping of the spatial organization of Caulobacter crescentus chromosome by chromosome conformation capture in conjunction with next-generation sequencing (Hi-C)
Chromosomes must be highly compacted and organized within cells, but how this is achieved in vivo remains poorly understood. We report the first use of Hi-C to map the structure of bacterial chromosomes at a high, unprecedented resolution. Analysis of Hi-C data and polymer modeling indicates that th...
ORGANISM(S): Caulobacter crescentus CB15 
2013-10-30 | E-GEOD-45966 | biostudies-arrayexpress
TranscriptomicsPhyK, PhyR, σT and σU regulons
The extracytoplasmic function sigma factor σT is the master regulator of general stress response in Caulobacter crescentus, and controls the expression of its paralogue σU. This work showed that PhyR and NepR act as positive and negative regulators, respectively, of σT expression and function. Bioch...
ORGANISM(S): Caulobacter crescentus NA1000 
2011-12-21 | E-GEOD-26977 | biostudies-arrayexpress
TranscriptomicsExpression analysis of Caulobacter crescentus NA1000 Plac::CCNA_00382 (ccrM) vs Caulobacter crescentus
Investigation of whole genome gene expression level changes in a Caulonacter crescentus NA1000 Plac::CCNA_00382 (ccrM) mutant, compared to the wild-type strain. The mutations engineered into this strain cause the CcrM DNA methyltransferase to be overexpressed and the chromosome to be constitutivel...
ORGANISM(S): Caulobacter crescentus NA1000 
2014-01-08 | E-GEOD-52722 | biostudies-arrayexpress
ProteomicsHipBA toxin-antitoxin system Caulobacter crescentus.
Antibiotic persistence is a transient phenotypic state during which a bacterium can withstand otherwise lethal antibiotic exposure or environmental stresses. In Escherichia coli, persistence is promoted by the HipBA toxin-antitoxin system. The HipA toxin functions as a serine/threonine kinase that i...
ORGANISM(S): Caulobacter crescentus (strain NA1000 / CB15N) 
2020-02-06 | PXD015525 | Pride
Caulobacter crescentus Phosphoproteome 
TranscriptomicsMeasuring the effects of hypoxia on transcription in Caulobacter crescentus
To test the effects of hypoxia on transcription in Caulobacter crescentus, we cultured cells in a New Brunswick bioreactor under controlled conditions. Prior to innoculation, the medium was bubbled with laboratory air at maximum flow and stirred at 300 rpm for 2 hours. After this period, the mediu...
ORGANISM(S): Caulobacter vibrioides 
2010-06-21 | E-GEOD-21127 | biostudies-arrayexpress
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