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ProteomicsOptimal Dissociation Methods Differ for N- and O-glycopeptides
Site-specific characterization of glycosylation requires intact glycopeptide analysis, and recent efforts have focused on how to best interrogate glycopeptides using tandem mass spectrometry (MS/MS). Beam-type collisional activation, i.e., higher-energy collisional dissociation (HCD), has been a val...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2020-07-20 | PXD017646 | Pride
Glycoproteomics N-glycopeptides Scehcd Ethcd O-glycopeptides Etd 
ProteomicsHuman serum N-linked intact glycopeptides LC-MS/MS
Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) in human serum is challenging due to the wide dynamic range of serum protein abundances, the lack of a complete serum N-Glycan database and the existence of non-specifical...
ORGANISM(S): Armoracia rusticana Homo sapiens (Human) Gallus gallus (Chicken) 
2020-04-21 | PXD015622 | Pride
N-linked intact glycopeptide Co-elution Spectral library search Human serum Isotopic distribution 
ProteomicsExtensive heterogeneity of glycopeptides in plasma revealed by deep glycoproteomic analysis
A number of plasma glycoproteins are clinically useful as biomarkers in a variety of diseases. Although thousands of proteins are present in plasma, >95% of the plasma proteome by mass is represented by only 22 proteins. This necessitates strategies to deplete the abundant proteins and enrich other...
ORGANISM(S): Homo sapiens (Human) 
2021-08-18 | PXD025414 | Pride
N-glycoproteomics Size exclusion chromatography Plasma glycoproteomics 
ProteomicsA Panel of Glycopeptides as Candidate Biomarkers for Early Diagnosis of NASH Hepatocellular Carcinoma Using a Stepped HCD Method and PRM Evaluation
A comprehensive screening of glycopeptides as candidate biomarkers from human serum for early diagnosis of NASH hepatocellular carcinoma using a stepped HCD method and PRM evaluation. Glycopeptides from vitronectin(VTNC) has been reported as biomarkers for NASH-related HCCs.
ORGANISM(S): Homo sapiens (Human) 
2021-05-10 | PXD025455 | Pride
Stepped-hcd ms/ms Nash hcc Large-scale glycopeptides analysis Biomarkers 
ProteomicsDirect glycan structure determination of intact N-linked glycopeptides by low-energy collision-induced association tandem mass spectrometry and predicted spectral library searching
Intact glycopeptide MS analysis to reveal site-specific protein glycosylation is an important frontier of proteomics. However, computational tools for analyzing MS/MS spectra of intact glycopeptides are still limited and not well-integrated into existing workflows. In this work, a novel computationa...
ORGANISM(S): Homo sapiens (Human) 
2016-08-17 | PXD002803 | Pride
Glycopeptide Lc-msms 
ProteomicsEvaluating the performance of photon- and electron-based fragmentation methods in Omnitrap-LCMS analysis of N-glycopeptides
To date, collision-induced dissociation and methods based on electron transfer dissociation are considered the standard approaches for the mass spectrometry analysis of N- and O-glycopeptides, respectively, allowing for identification of both peptide and glycan compositions. In recent years, alterna...
ORGANISM(S): Homo sapiens (Human) 
2026-09-07 | PXD071023 | Pride
Glycopeptides Omnitrap Ecd Uvpd Eid 
ProteomicsCharacterization of sialylated N- and O-glycopeptides from complex proteomes on an Orbitrap Fusion Tribrid mass spectrometer by Isotope Targeted Glycoproteomics (IsoTaG). Application to azido and alkynyl sugars
Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation. Correlation of the intact glycoform to the protein attachment site is a critical step to assign functional roles to specific glycoproteins. Isotope targeted...
ORGANISM(S): Homo sapiens (Human) 
2017-02-22 | PXD004302 | Pride
Lc-ms/ms Tissue culture Glycoproteomics Metabolic labeling 
ProteomicsElectron-based dissociation is needed for O-glycopeptides derived from OpeRATOR proteolysis
The recently described O-glycoprotease OpeRATOR presents exciting opportunities for O-glycoproteomics. This bacterial enzyme purified from A. muciniphila cleaves N-terminally to serine and threonine residues that are modified with (preferably asialylated) O-glycans, providing orthogonal cleavage rel...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2020-11-03 | PXD020077 | Pride
Glycoproteomics O-glycosites Operator Protease Electron transfer dissociation 
ProteomicsATP-Coated Dual-functionalized Titanium (IV) IMAC Material for Simultaneous Enrichment and Separation of Glycopeptides and Phosphopeptides
Protein glycosylation and phosphorylation are two of the most common post-translational modifications (PTMs), which plays an important role in many biological processes. However, low abundance and poor ionization efficiency of phosphopeptides and glycopeptides make direct MS analysis challenging. Pr...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-07-20 | PXD029775 | Pride
Phosphoproteomics Lc-ms/ms Glycoproteomics 
ProteomicsPseudaminic acid containing glycopeptides enrichment of Acinetobacter baumannii strains BAL062
Enrichment of Pseudaminic acid containing glycopeptides of Acinetobacter baumannii strains BAL062
ORGANISM(S): unclassified Acinetobacter calcoaceticus/baumannii complex 
2025-10-22 | PXD046859 | Pride
Pseudaminic acid containing glycopeptides Acinetobacter baumannii 
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