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ProteomicsOptimal Dissociation Methods Differ for N- and O-glycopeptides
Site-specific characterization of glycosylation requires intact glycopeptide analysis, and recent efforts have focused on how to best interrogate glycopeptides using tandem mass spectrometry (MS/MS). Beam-type collisional activation, i.e., higher-energy collisional dissociation (HCD), has been a val...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2020-07-20 | PXD017646 | Pride
Glycoproteomics N-glycopeptides Scehcd Ethcd O-glycopeptides Etd 
ProteomicsHuman serum N-linked intact glycopeptides LC-MS/MS
Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) in human serum is challenging due to the wide dynamic range of serum protein abundances, the lack of a complete serum N-Glycan database and the existence of non-specifical...
ORGANISM(S): Armoracia rusticana Homo sapiens (Human) Gallus gallus (Chicken) 
2020-04-21 | PXD015622 | Pride
N-linked intact glycopeptide Co-elution Spectral library search Human serum Isotopic distribution 
ProteomicsLC-MS/MS analysis of maize seedling leaf bases
1 g samples of maize seedling leaf bases were collected from the wild-type (Zheng58) plants and the mutants (bzu3-2). we undertook glycoproteomics analyses of the N-glycans. Based on the libraries of maize protein group and plant N-glycosylation modification in the Uniprot database, we identified 2,...
ORGANISM(S): Zea mays (Maize) 
2023-07-24 | PXD041642 | Pride
N-glycopeptides Maize Lc-ms/ms 
ProteomicsProfiling the level of N-glycan structural heterogeneity in Arabidopsis N-glycoproteins
In this study, we use hydrophilic enrichment, high-resolution tandem mass spectrometry with complimentary and triggered fragmentation to profile Arabidopsis N-glycopeptides. A total of 492 N-glycosites were identified from 324 Arabidopsis proteins with extensive N-glycan structural heterogeneity rev...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-12-18 | PXD006270 | Pride
Arabidopsis Glycopeptides N-glycosylation 
ProteomicsHuman Bone Morphogenetic Protein Receptor 2 (BMPR2) N-Glycopeptides
Trypsin,Glu-C and trypsin-Glu-C N-glycopeptides spanning all three putative BMPR2 N-glycosylation sites (N29, N84, and N100) were observed. Glycopeptide analyses revealed N-glycan compositions consistent with hybrid and complex glycoforms with abundant fucosylation and sialylation.
ORGANISM(S): Homo sapiens (Human) 
2026-07-06 | PXD066494 | Pride
N-glycopeptide Scehcd Bmpr2 Ethcd N-glycosylation Hek293 
ProteomicsDiversity matters: Optimal collision energies for tandem mass spectrometric analysis of a large set of N-glycopeptides - part 2
a) Ratio of the lower and higher energy component. Tests were done on the mixture of AGP, fetuin and transferrin digests using 2.2 pmol from all glycoproteins in each run. Five different high CE setting was used which were 50%, 75%, 100%, 125% and 150% of the original method of Hinneburg et al. Thes...
ORGANISM(S): Bos Taurus (ncbitaxon:9913) Homo Sapiens (ncbitaxon:9606) 
2022-08-26 | MSV000090218 | MassIVE
ProteomicsComparison of Intact Glycopeptide Enrichment Methods Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography
Heterogeneity of protein glycosylation poses great challenge for analysis that is key to un-puzzle systems glycobiology in diseases. Resolving this conundrum requires global enrichment of glycopeptides for identification and quantitation. To this aim, hydrophilic interaction chromatography (HILIC) h...
ORGANISM(S): Homo sapiens (Human) 
2017-10-19 | PXD005145 | Pride
Glycoproteomics Hilic Sax Quantitation O-linked 
UnknownClassification of Tandem Mass Spectra for Identification of N- and O-linked Glycopeptides.
Not available
S-EPMC5116676 | biostudies-literature
ProteomicsBloodstream infection N-glycoproteomics
Here we have performed targeted quantitative N-glycoproteomics from plasma samples of patients with confirmed positive blood culture together with age and sex matched febrile controls with negative blood culture reports. Three hundred and sixty eight potential N-glycopeptides were quantified by mass...
ORGANISM(S): Homo sapiens (Human) 
2018-04-09 | PXD009048 | Pride
Sepsis Human plasma Bloodstream infection N-glycoproteomics 
ProteomicsIntact N-glycopeptides in Serum Haptoglobin between HCC and Cirrhosis
A workflow for differential analysis of the microheterogeneity of site-specific intact N-glycopeptides of serum haptoglobin between early hepatocellular carcinoma (HCC) and liver cirrhosis has been developed.
ORGANISM(S): Homo sapiens (Human) 
2018-10-25 | PXD011239 | Pride
Human Serum Haptoglobin Lc-ethcd-ms/ms Liver 
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