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ProteomicsApplicability of chromatographic co-elution for antibiotic research
Identification of the molecular target is crucial for evaluating novel antibiotics. To support target identification, a label-free method based on chromatographic co-elution (TICC) has been developed previously. In TICC, an alteration in the elution profile of the target-bound drug compared to free ...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2020-10-01 | PXD017640 | Pride
Essential proteins Bacillus subtilis Protein complexes Protein-protein interaction 
ProteomicsMultiple solvent elution, a method to counter the effects of Co-Elution and Ion Suppression of peptides in LC-MS analysis.
The abundance of proteins in the cellular proteome ranges from millions of copies to one copy per cell. This wide dynamic range represents a major challenge in proteomic analysis. On average, any human cell type expresses up to approximately 10.000 different protein coding genes that are used to syn...
ORGANISM(S): Homo sapiens (Human) 
2019-06-28 | PXD011908 | Pride
Proteome coverage Co-elution Lc-ms Proteomics Solvents Ion suppression 
UnknownImproved elution conditions for native co-immunoprecipitation.
Not available
S-EPMC3063181 | biostudies-literature
UnknownA rapid and accurate approach for prediction of interactomes from co-elution data (PrInCE).
Not available
S-EPMC5654062 | biostudies-literature
ProteomicsThe HAPSTR2 retrogene buffers stress signaling and resilience in mammals
Standard anti-FLAG magnetic beads for co-immunoprecipitations, elution in 2x SDS sample buffer, loaded into a stacking gel and then in-gel digested
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD038642 | Pride
Hapstr2 Hapstr1 Huwe1 
UnknownIntroduction of Temperature-Gradient Elution in Three-Dimensional Correlation Thermal Field-Flow Fractionation with Quintuple Detection for Monitoring Compositional Dynamics of Ultrahigh-Molar-Mass Poly(styrene-<i>co</i>-maleic anhydride).
Not available
S-EPMC12825381 | biostudies-literature
ProteomicsCarrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering
We added increasing amounts of E coli carrier proteome to BSA, our moch recombinant protein to model interference of high-background BSA peaks with smaller peaks of E coli HCPs, using MS2 and MS3 methods to show co-fragmentation and co-elution based on similar m/z ratio. We created an interference d...
ORGANISM(S): Escherichia coli 
2026-01-12 | PXD050183 | Pride
Host cell proteins (hcps) Peptide-spectrum matches Tmt Co-elution Lc-ms Multiplexed proteomics Tryptic digest Interference Carrier proteome Data dependent acquisition Co-fragmentation 
ProteomicsSystem-wide profiling of protein-complexes via size exclusion chromatography– mass spectrometry (SEC-MS)
In living cells most proteins are organized in stable or transient functional assemblies known as protein complexes, which control a multitude of vital cellular processes such as cell cycle progression, metabolism, and signal transduction. System-wide workflows for analysis of protein complexes usin...
ORGANISM(S): Homo sapiens (Human) 
2022-05-06 | PXD018033 | Pride
Protein complex analysis Sec-ms Protein co-elution profiling 
ProteomicsSEC-SWATH-MS HEK293 - Complex-centric proteome profiling by SEC-SWATH-MS
Increasingly, biochemical co-fractionation-based approaches are used to study interactomes and protein complexes at high throughput. The devised methods facilitate the qualitative assignment and prediction of hundreds of putative cellular assemblies in one experiment and without dependency on geneti...
ORGANISM(S): Homo sapiens (Human) 
2019-01-11 | PXD007038 | Pride
Size exclusion chromatography Mass spectrometry Swath Protein complexes 
MetabolomicsAutomated Label-free Quantification of Metabolites from Liquid Chromatography–Mass Spectrometry Data (Plasma)

Liquid chromatography coupled to mass spectrometry (LC-MS) has become a standard technology in metabolomics. In particular, label-free quantification based on LC-MS is easily amenable to large-scale studies and thus well suited to clinical metabolomics. Large-scale studies, however, require autom...

2015-12-16 | MTBLS234 | MetaboLights
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