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ProteomicsOptimal Dissociation Methods Differ for N- and O-glycopeptides
Site-specific characterization of glycosylation requires intact glycopeptide analysis, and recent efforts have focused on how to best interrogate glycopeptides using tandem mass spectrometry (MS/MS). Beam-type collisional activation, i.e., higher-energy collisional dissociation (HCD), has been a val...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2020-07-20 | PXD017646 | Pride
Glycoproteomics N-glycopeptides Scehcd Ethcd O-glycopeptides Etd 
ProteomicsA Combinatorial Enzymatic Approach for Identifying O-glycopeptides
Proteins glycosylation is primarily characterized as N-glycosylation or O-glycosylation. Recognition of the consensus N-glycosylation sequon (N-X-S/T/C) has enabled the mapping of the glycosite occupancy of intact glycopeptides, whereas O-glycosylation consensus sequons do not exit, making the chara...
ORGANISM(S): Homo sapiens (Human) 
2024-04-25 | PXD042701 | Pride
Rax-based enrichment O-glycosites O-glycosylation Impa Scehcd-based ms/ms 
ProteomicsSite-specific Extraction of O-linked glycopeptides (EXoO) Maps Landscape of In Vivo O-linked Glycoproteome
Protein glycosylation is one of the most common protein modifications and plays essential roles in biology and therapeutics. However, the analysis of in vivo O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of technology. Here, a chemoenzymatic...
ORGANISM(S): Homo sapiens (Human) 
2018-12-05 | PXD009476 | Pride
Site-specific Glycosylation Exoo In vivo O-linked 
ProteomicsElectron-based dissociation is needed for O-glycopeptides derived from OpeRATOR proteolysis
The recently described O-glycoprotease OpeRATOR presents exciting opportunities for O-glycoproteomics. This bacterial enzyme purified from A. muciniphila cleaves N-terminally to serine and threonine residues that are modified with (preferably asialylated) O-glycans, providing orthogonal cleavage rel...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2020-11-03 | PXD020077 | Pride
Glycoproteomics O-glycosites Operator Protease Electron transfer dissociation 
ProteomicsLarge-scale and Site-specific Mapping of Intact O-glycans on Glycopeptides with IMPa
We employed the newly available IMPa O-glycoprotease from Pseudomonas aeruginosa for O-glycoproteomics analysis of cultured cells and tissues. The glycopeptides were extracted, purified, and conjugated to a solid support before an enzymatic cleavage by IMPa. O-glycopeptides were analyzed by EThcD, w...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-08-28 | PXD037415 | Pride
O-glycoproteomics  impa  vva  rax  tn antigen 
ProteomicsA broad-specificity O-glycoprotease that enables improved analysis of glycoproteins and glycopeptides containing intact complex O-glycans
Analysis of mucin type O-glycans linked to serine/threonine of glycoproteins is technically challenging, in part, due to a lack of effective enzymatic tools that enable their analysis. Recently, several O-glycan-specific endoproteases that can cleave the protein adjacent to the appended glycan have ...
ORGANISM(S): Homo sapiens (Human) 
2022-01-05 | PXD029534 | Pride
G-csf Cd45 
ProteomicsComparison of Intact Glycopeptide Enrichment Methods Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography
Heterogeneity of protein glycosylation poses great challenge for analysis that is key to un-puzzle systems glycobiology in diseases. Resolving this conundrum requires global enrichment of glycopeptides for identification and quantitation. To this aim, hydrophilic interaction chromatography (HILIC) h...
ORGANISM(S): Homo sapiens (Human) 
2017-10-19 | PXD005145 | Pride
Glycoproteomics Hilic Sax Quantitation O-linked 
ProteomicsMapping and quantification of over 2,000 O-linked glycopeptides in activated human T cells with isotope-targeted glycoproteomics
Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example. Mounting evidence indicates that the modification of proteins by O-linked Nacetylglucosamine (O-GlcNAc), the o...
ORGANISM(S): Homo sapiens (Human) 
2018-01-25 | PXD004559 | Pride
Glycoproteomics Mass spectrometry O-glcnac T cells O-linked n-acetylglucosamine 
ProteomicsComparison of Intact Glycopeptide Enrichment Methods Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography
Heterogeneity of protein glycosylation poses great challenge for analysis that is key to un-puzzle systems glycobiology in diseases. Resolving this conundrum requires global enrichment of glycopeptides for identification and quantitation. To this aim, hydrophilic interaction chromatography (HILIC) h...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-07-02 | MSV000082566 | MassIVE
UnknownDirect Identification of O-Glycopeptides by Low-Temperature Assisted Nanopore Technique.
Not available
S-EPMC12410930 | biostudies-literature
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