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GenomicsHighly multiplexed mRNA quantitation with CRISPR-Cas13
Highly multiplexed mRNA quantitation with CRISPR-Cas13
PRJNA1222449 | ENA
ProteomicsInfluence of digestion protocol in Protein Quantitation
Quantitative determination of absolute and relative protein amounts is an essential requirement for most current bottom-up proteomics applications, but protein quantitation estimates are affected by several sources of variability such as sample preparation, mass spectrometric acquisition, and data a...
ORGANISM(S): Escherichia coli 
2014-07-30 | PXD001187 | Pride
Protein quantitation Digestion protocols Fasp 
TranscriptomicsHighly multiplexed mRNA quantitation with CRISPR-Cas13
RNA quantitation tools are often either high-throughput or cost-effective, but rarely are they both. Existing methods can profile the transcriptome at great expense or are limited to quantifying a handful of genes by labor constraints. A technique that permits more throughput at a reduced cost could...
ORGANISM(S): Homo sapiens 
2025-10-31 | GSE289345 | GEO
TranscriptomicsAnalysis of RNA quantitation in formaldehyde-fixed cells
Fluorescence-activated cell sorting (FACS) is a sensitive and valuable technique to characterize cellular subpopulations and great advances have been made using this approach. Cells are often fixed with formaldehyde prior to the sorting process to preserve cell morphology and maintain the expression...
ORGANISM(S): Homo sapiens 
2013-09-09 | GSE50690 | GEO
ProteomicsSysQuan: repurposing SILAC mice for affordable absolute quantitation of the human proteome
Relative quantitation, used by most MS-based proteomics laboratories to determine protein fold-changes, requires that samples are processed and analyzed together to enable best comparability through minimizing batch differences. This limits the detection of subtle but relevant changes in heterogeneo...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-06-16 | PXD057266 | Pride
Proteomics Silac Stable isotope Targeted proteomics Absolute quantitation 
TranscriptomicsAnalysis of RNA quantitation in formaldehyde-fixed cells
Fluorescence-activated cell sorting (FACS) is a sensitive and valuable technique to characterize cellular subpopulations and great advances have been made using this approach. Cells are often fixed with formaldehyde prior to the sorting process to preserve cell morphology and maintain the expression...
ORGANISM(S): Homo sapiens 
2013-09-09 | E-GEOD-50690 | biostudies-arrayexpress
ProteomicsQuantitation of ATIs in Wheat by LC-MS/MS – iTRAQ
A selection of ATIs was silenced in the common wheat cultivar Bobwhite and durum wheat cultivar Svevo by RNAi and gene editing, respectively, in order to reduce the amounts of ATIs. The controls and silenced lines were analyzed after digestion to peptides by LC-MS/MS with different approaches to eva...
ORGANISM(S): Triticum aestivum (Wheat) Triticum durum 
2022-10-13 | PXD034478 | Pride
Amylase/trypsin inhibitor  wheat  crsipr-cas9  rnai 
GenomicsAn integrated 5'- and 3'-end RNA sequencing platform for high-throughput transcriptome quantitation and annotation
An integrated 5'- and 3'-end RNA sequencing platform for high-throughput transcriptome quantitation and annotation
PRJNA205109 | ENA
ProteomicsLabel Free MS quantitation of rice Oryza sativa (control vs drought)
Label Free MS quantitation of rice Oryza sativa (control vs drought)
ORGANISM(S): Oryza sativa (Rice) 
2024-10-17 | PXD036637 | Pride
Oryza sativa Lc-ms Label-free quantitation 
ProteomicsQuantitation using pyBinder
Novel method of quantitation for targeted sequencing of peptides of interest in affinity selection-mass spectrometry experiments
ORGANISM(S): Mus musculus (Mouse) 
2024-11-21 | PXD057975 | Pride
Affinity selection-mass spectrometry Pybinder Label-free quantitation 
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