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ProteomicsUVPD applied to phosphoproteomics
UV-photodissociation was incorporated into a phosphoproteomic workflow for the analysis of peptide PTMs.
ORGANISM(S): Homo sapiens (Human) synthetic construct 
2017-08-08 | PXD003109 | Pride
Uvpd  phosphoproteomics 
ProteomicsOnline Weak Cation Exchange-HILIC × Reversed Phase LC UVPD-HRMS of intact HeLa Histones
Intact HeLa core Histones were analyzed using an online two-dimensional liquid chromatographic separation as well as one dimensional LC (RPLC and WCX-HILIC) coupled with UVPD-MS
ORGANISM(S): Homo sapiens (Human) 
2018-09-19 | PXD008978 | Pride
Histones Top-down proteomics Online 2dlc Uvpd 
ProteomicsUVPD of peptides modified with N-terminal UV-absorbing chromophores
Ultraviolet photodissociation (UVPD) has emerged as a powerful alternative to conventional collision-induced dissociation (CID) for peptide and protein sequencing in mass spectrometry–based proteomics. However, UVPD efficiency depends on the presence of UV-absorbing chromophores within analytes.The ...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD075227 | Pride
Chromophores Lc-ms Proteomics Nhs chemistry Uvpd 
ProteomicsLight-induced orthogonal fragmentation of crosslinked peptides
Crosslinking mass spectrometry provides pivotal information on the structure and interaction of proteins. MS-cleavable crosslinkers are regarded as a cornerstone for the analysis of complex mixtures. Yet they fragment under similar conditions as peptides, leading to mixed fragmentation spectra of th...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2023-08-28 | PXD040267 | Pride
Clms Cleavable crosslinker Crosslinking Uvpd 
ProteomicsUltraviolet Photodissociation of ESI and MALDI generated protein ions on a Q-Exactive mass spectrometer
UVPD was implemented on an Orbitrap Q-Exactive plus equipped with a ESI/EP-MALDI. UVPD of MALDI generated ions was benchmarked against MALDI-ISD, MALDI-HCD and ESI-UVPD. MALDI UVPD outperformed MALDI HCD and ISD efficiently sequencing small proteins ions.
ORGANISM(S): Homo sapiens (Human) Equus caballus (Horse) 
2019-01-24 | PXD011526 | Pride
Ms/ms Uvpd Q-exactive Maldi 
ProteomicsUltraviolet Photodissociation of tryptic peptide backbones at 213 nm
We analyzed the backbone fragmentation behavior of tryptic peptides of a four protein mixture and of E. coli lysate subjected to Ultraviolet Photodissociation (UVPD) at 213 nm on a commercially available UVPD-equipped tribrid mass spectrometer. We obtained 15,178 high-confidence peptide-spectrum mat...
ORGANISM(S): Homo sapiens (Human) Oryctolagus cuniculus (Rabbit) Escherichia coli Gallus gallus (Chicken) Equus caballus (Horse) 
2020-05-07 | PXD018176 | Pride
Lc-msms E. coli Ovotransferrin Fusion lumos Dual ms2 Uvpd 213 nm Hsa Myoglobin Creatine kinase 
ProteomicsEnhanced Characterization of Lysine-Linked Antibody Drug Conjugates Enabled by Middle-Down Mass Spectrometry
HCD Triggered UVPD and EThcD files for characterization of trastuzumab-emtansine (T-DM1)
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-03 | MSV000092585 | MassIVE
ProteomicsIntegrating Alternative Fragmentation Techniques into Standard LC-MS Workflows Using a Single Deep Learning Model Enhances Proteome Coverage
We built and characterised a mass spectrometer capable of performing CID (both beam type and resonant type), UVPD, EID and ECD in an automated fashion during an LCMS type experiment. We exploited this ability to generate large datasets through multienzyme deep proteomics experiments for characterisa...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Homo sapiens (Human) Escherichia coli 
2026-03-13 | PXD065289 | Pride
Ecd Eid Uvpd 
ProteomicsApplication of 193 nm UVPD for phosphoproteomic analysis of HeLa and HCC70
Application of 193 nm ultraviolet photodissociation (UVPD) for phosphoproteomics analysis of IMAC enriched HeLa and HCC70 lysates. HCC70 cells were either maintained in full media or serum starved for 6 hours prior to lysis. Each sample was analyzed by three UVPD and three HCD technical replicates u...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-04-04 | MSV000079626 | MassIVE
ProteomicsComplementary CID and 351 nm UVPD tandem mass spectra enable effective de novo sequencing
This dataset accompanies a publication in which we present a strategy for acquisition and effective de novo peptide sequencing of complementary CID and 351 nm ultraviolet photodissociation (UVPD) MS/MS pairs. E. coli whole cell lysate is carbamylated to block lysine side chains, digested with trypsi...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) 
2017-03-06 | MSV000080603 | MassIVE
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