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This experiment is part of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th Framework Programme of the European Union, http://www.fungenes.org). The experiment was conducted at University of Cologne, Cologne, Germany. Goal of the experiment is ...
ORGANISM(S): Mus musculus 
The pro-angiogenic potential of CD31+ cell populations isolated from human ADRCs was firmly established in ex vivo and in vitro assays. To understand the cellular identity of these CD31+ ADRCs in detail, we performed high-resolution single-cell RNA-sequencing (scRNA-seq) of CD31+ enriched cells from...
ORGANISM(S): Homo sapiens 
In order to assess whether there were differences in gene expression between the polyclonal CD31+ and the polyclonal CD31- cell populations of adipose-derived adult stem cells (ADASCs), we wanted to identify possible genes that were differentially expressed between these two cell populations. To tha...
ORGANISM(S): Homo sapiens 
A major challenge in realizing regenerative treatment using freshly isolated Adipose Derived Regenerative Cells (ADRCs) is the cellular heterogeneity and donor variability. Ex vivo expanded ADRCs (=ASCs) are available at larger quantities and represent a more homogeneous population suitable for allo...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD040388 | Pride
In order to assess whether culturing adipose-derived adult stem cells (ADASCs) affect their gene expression (see Sample Growth Condition Protocol), we wanted to identify possible genes that were differentially expressed between cultured polyclonal CD31- ADASCs and freshly isolated (uncultured) polyc...
ORGANISM(S): Homo sapiens 
To investigate the heterogeneity of lung stromal cells and identify the specific lung stromal subset, we performed single cell RNA-sequencing (scRNA-seq) on lung stromal cells (CD45-CD31-CD326-). Around 6800 cells were captured using the 10x Chromium technology.
ORGANISM(S): Mus musculus 
Adult mice hearts contain a population of resident mesenchymal stem cell (MSC)-like cells called cardiac colony forming units-fibroblast (cCFU-F). The cCFU-F are housed in a population of non-muscle cardiac cells that are Pdgfra+/Sca1+/Cd31- (S+P+ fraction). The goal of this experiment was to profil...
ORGANISM(S): Mus musculus 
Hematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial, and hematopoietic lineages, it remains unknown whether the mammalian ...
ORGANISM(S): Mus musculus 
Gene expression signatures of splenic CD31+ACKR4+ and CD31+ACKR4- endothelial cells from Ackr4GFP/wt mice
Naive CD4 T cells defined as (TCRab+ CD4+ CD45RA+ CD62L+ CD127+) were further divided by CD25 and CD31 expression with FACS-based flow sorting. Subsequently, gene expression levels of each subset were assessed by using transcription arrays.
ORGANISM(S): Homo sapiens 
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