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His6-Cdc48a and associated proteins purified from H. volcanii. A) SDS- PAGE of proteins purified by Ni2+ -chromatography. H. volcanii carrying empty vector control (H1209-pJAM202c, lane 1) and ectopically expressing His6-Cdc48a (H1209- pJAM1409, lane 2). Red bars indicate regions of gel excised. B)...
ORGANISM(S): Haloferax volcanii DS2 
2023-06-22 | PXD019906 | Pride
H. volcanii Cdc48a, RecJ3/4 and RNase J associate with Ubl-coated beads. A) Strategy to isolate H. volcanii proteins that bind agarose beads charged with monomeric Ubl (vs. BSA) as bait. Cell lysate was from triplicate cultures of H. volcanii NH02-pJAM957. B) Left, Non-reducing SDS-PAGE of H. volcan...
ORGANISM(S): Haloferax volcanii DS2 
2023-06-22 | PXD019897 | Pride
RecJ4 association with RecJ3, RNase J and Cdc48A investigated by pullingown strep tagged-RecJ4 with its partner proteins
ORGANISM(S): Haloferax volcanii DS2 
2023-06-22 | PXD019933 | Pride
detecting core complex formation for RecJ3/4 and RNase J form a core complex.
ORGANISM(S): Haloferax volcanii DS2 
2023-06-22 | PXD019932 | Pride
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