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ProteomicsProteins associated with Cdc48A identified by LC-MS\MS data
His6-Cdc48a and associated proteins purified from H. volcanii. A) SDS- PAGE of proteins purified by Ni2+ -chromatography. H. volcanii carrying empty vector control (H1209-pJAM202c, lane 1) and ectopically expressing His6-Cdc48a (H1209- pJAM1409, lane 2). Red bars indicate regions of gel excised. B)...
ORGANISM(S): Haloferax volcanii DS2 
2023-06-22 | PXD019906 | Pride
Cdc48a Hvo_2380 
ProteomicsCdc48A pulldown from an amino lonk column baited with SAMP1
H. volcanii Cdc48a, RecJ3/4 and RNase J associate with Ubl-coated beads. A) Strategy to isolate H. volcanii proteins that bind agarose beads charged with monomeric Ubl (vs. BSA) as bait. Cell lysate was from triplicate cultures of H. volcanii NH02-pJAM957. B) Left, Non-reducing SDS-PAGE of H. volcan...
ORGANISM(S): Haloferax volcanii DS2 
2023-06-22 | PXD019897 | Pride
Cdc48a Samp1 Hvo_2380 
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