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MetabolomicsMetabolic Profiling of Escherichia coli-Based Cell-Free Expression Systems for Process Optimization
Biotechnology has transformed the production of various chemicals and pharmaceuticals due to its efficient and selective processes, but it is inherently limited by its use of live cells as 'biocatalysts.' Cell-free expression (CFE) systems, which use a protein lysate isolated from whole cells, have ...
2020-06-02 | MTBLS1079 | MetaboLights
MetabolomicsMycelium of Diaporthe nobilis treated with 2% cell-free supernatant (CFS) from Bacillus velezensis A4 for 12 hours

Mycelia of Diaporthe nobilis(50 mg) treated with CFS were collected,Each sample was homogenized in 400 µL of pre-cooled 80% methanol aqueous solution containing 0.02 mg/mL L-2-chlorophenylalanine (internal standard). The mixture was ground and subjected to ultrasonication at 4 °C for 30 min. Afte...

2025-11-19 | MTBLS13351 | MetaboLights
MetabolomicsDNA-PKcs drives CKD progression by activating TAF7/RAPTOR/mTORC1 signaling-mediated metabolic reprogramming

Kidney injury initiates epithelial dedifferentiation and myofibroblast activation during the progression of chronic kidney disease (CKD). Herein, we found that the expression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) was significantly increased in the kidney tissues of both CKD...

2023-01-25 | MTBLS5971 | MetaboLights
GenomicsTissue-specific cell-free DNA degradation quantifies circulating tumor DNA burden
Profiling of circulating tumor DNA (ctDNA) may offer a non-invasive approach to monitor disease progression. Here, we developed a quantitative method, exploiting local tissue-specific cell-free DNA (cfDNA) degradation patterns, that accurately estimates ctDNA burden independent of genomic aberration...
EGAS00001004657 | EGA
GenomicsLong cell-free DNA molecules in maternal plasma
We revealed a large population of long cell-free DNA molecules (up to 23,635 bp in length) in maternal plasma and developed an approach which leveraged the abundance of CpG sites on long molecules to deduce the tissue of origin of individual plasma DNA molecules based on single-molecule methylation ...
EGAS00001005515 | EGA
GenomicsLong-read sequencing for cell-free DNA analysis (human)
In this study, we compared the two long-read sequencing platforms, namely the single-molecule real-time sequencing by Pacific Biosciences and nanopore sequencing by Oxford Nanopore Technologies, for the analysis of cell-free DNA from plasma. Cell-free DNA from plasma samples of 31 pregnant women at ...
EGAS00001006328 | EGA
GenomicsFFPE, buffycoat and cell free DNA from N. German esophagus cancer patients - 2021
We recruited 28 patients and sequenced samples from their FFPE tissue, full blood buffy coat and plasma cell free DNA from diagnosis and other, longitudinal time points. The cell free DNA was sequenced ultra-deeply. The IDT PanCancer panel was used for targeted enrichment. Aims: detection of primar...
EGAS00001006813 | EGA
GenomicsCell-free DNA TAPS for early cancer detection
Multimodal, genome-wide characterization of epigenetic and genetic information in circulating cell-free DNA (cfDNA) could enable more sensitive early cancer detection. However, due to technological challenges associated with DNA methylation sequencing in low input cfDNA samples, most studies have be...
EGAS00001004962 | EGA
TranscriptomicsAndrogen receptor gene aberrations in circulating cell-free DNA: biomarkers of therapeutic resistance and response in castration-resistant prostate cancer
aCGH experiment on cell-free DNA collected from the plasma of patients with castration-resistant prostate cancer. No replicates. castration-resistant prostate cancer vs male reference DNA
ORGANISM(S): Homo sapiens 
2015-03-09 | E-GEOD-61134 | biostudies-arrayexpress
GenomicsDNA demethylation triggers cell free DNA release in colorectal cancer cells
DNA demethylation triggers cell free DNA release in colorectal cancer cells
PRJEB78621 | ENA
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