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ProteomicsProteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling
Despite the growing interest in using chemical genetics in plant research, small-molecule target identification remains a major challenge. The cellular thermal shift assay coupled with high-resolution massspectrometry (CETSA MS) that monitors changes in the thermal stability of proteins caused by th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-04 | PXD030309 | Pride
Brassinosteroids Auxin Chemical genetics Cellular thermal shift assay 
ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
ProteomicsInvestigation of cellular response to the HSP90 inhibition in human cells through thermal proteome profiling
Heat shock proteins are responsible for protein folding in cells. HSP90 is one of the most important chaperones in human cells, and inhibiting HSP90 is a potential strategy for cancer therapy. Multiple HSP90 inhibitors have been in clinical trials. However, none of them has been approved for disease...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD037575 | Pride
Heat shock protein 90 Hsp90 inhibitors And protein abundance change Thermal proteome profiling Cell response Protein thermal stability shift 
TranscriptomicsEZH2 inhibitor efficacy in non-Hodgkin lymphoma does not require suppression of H3K27 mono-methylation [RNA-Seq]
Here we report the discovery of highly potent and selective EZH2 small molecule inhibitors, their validation by a cellular thermal shift assay, their application across a large lymphoma cell panel and their efficacy in GCBDLBCL xenograft models. RNA-seq of KARPAS-422 cell line RNA, in duplicate, tre...
ORGANISM(S): Homo sapiens 
2015-04-13 | E-GEOD-62056 | biostudies-arrayexpress
UnknownRapid Evaluation of Small Molecule Cellular Target Engagement with a Luminescent Thermal Shift Assay.
Not available
S-EPMC8366000 | biostudies-literature
UnknownHigh-Throughput Cellular Thermal Shift Assay Using Acoustic Transfer of Protein Lysates.
Not available
S-EPMC10026039 | biostudies-literature
UnknownHigh-Throughput Detection of Ligand-Protein Binding Using a SplitLuc Cellular Thermal Shift Assay.
Not available
S-EPMC9502016 | biostudies-literature
UnknownA widely-applicable high-throughput cellular thermal shift assay (CETSA) using split Nano Luciferase.
Not available
S-EPMC6013488 | biostudies-literature
UnknownReal-Time Cellular Thermal Shift Assay to Monitor Target Engagement.
Not available
S-EPMC9486815 | biostudies-literature
UnknownA cellular thermal shift assay for detecting amino acid sites involved in drug target engagement.
Not available
S-EPMC9168144 | biostudies-literature
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