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TranscriptomicsTelomeric repeats facilitate CENP-A incorporation via telomere binding proteins
The histone H3 variant, CENP-ACnp1, is normally assembled upon canonical centromeric sequences, but there is no apparent obligate coupling of sequence and assembly, suggesting that centromere location can be epigenetically determined. To explore the tolerances and constraints on CENP-ACnp1 depositio...
ORGANISM(S): Schizosaccharomyces pombe 
2013-08-22 | E-GEOD-46427 | biostudies-arrayexpress
TranscriptomicsFactors that promote H3 chromatin integrity during transcription prevent promiscuous deposition of CENP-A(Cnp1) in fission yeast
Specialized chromatin containing CENP-A nucleosomes instead of H3 nucleosomes is found at all centromeres. However, the mechanisms that specify the locations at which CENP-A chromatin is assembled remain elusive in organisms with regional, epigenetically regulated centromeres. It is known that norma...
ORGANISM(S): Schizosaccharomyces pombe 
2015-02-19 | E-GEOD-66106 | biostudies-arrayexpress
GenomicsGenome-wide maps of WT and over-expressing CenH3/CENP-A in Human HeLa S3 cells
Centromeres are essential to ensure proper chromosome segregation in eukaryotes. Their definition relies on the presence of a centromere-specific H3 histone variant CenH3, known as CENP-A in mammals. Its overexpression in aggressive cancers raises questions concerning its effect on chromatin dynamic...
ORGANISM(S): Homo sapiens 
2014-02-12 | E-GEOD-42951 | biostudies-arrayexpress
GenomicsCENP-A ChIP-seq of MCF10-2A cells to study CENP-A localization following reversal from high to basal CENP-A expression
CENP-A, the centromeric histone H3 variant, is essential for the proper segregation of human chromosomes to daughter cells. High CENP-A expression leads to the misincorporation of CENP-A in non-centromeric chromatin and can promote an epithelial-mesenchymal transition (EMT). Here, we explored how C...
ORGANISM(S): Homo sapiens 
2026-04-24 | E-MTAB-16636 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA-Seq to study the effects of acute and chronic CENP-A overexpression depending on p53 status
We performed single-cell RNA-Seq to determine the effects of CENP-A overexpression and p53 status on cell fate over time. We established clonal MCF10-2A TetOn-CENPA-FLAG-HA cell lines where CENP-A can be reversibly induced by Doxycycline (Dox) treatment. We compared prolonged, continuous CENP-A over...
ORGANISM(S): Homo sapiens 
2021-03-01 | E-MTAB-9861 | biostudies-arrayexpress
GenomicsCENP-A and H3.3 ChIP-seq of MCF10-2A cells to study how high CENP-A levels promote EMT
CENP-A, the centromeric histone H3 variant, is essential for the proper segregation of human chromosomes to daughter cells. High CENP-A expression leads to the misincorporation of CENP-A in non-centromeric chromatin and can promote an epithelial-mesenchymal transition (EMT). Here, we explored how C...
ORGANISM(S): Homo sapiens 
2026-04-24 | E-MTAB-15579 | biostudies-arrayexpress
GenomicsThe N-terminal Tail of CENP-A Confers Epigenetic Stability to Centromeres via the CENP-T Branch of the CCAN in Fission Yeast
We employ the well-studied fission yeast centromere to investigate the function of the CENP-A (Cnp1) N-tail. We show that alteration of the N-tail did not affect Cnp1 loading at centromeres, outer kinetochore formation, or spindle checkpoint signaling, but nevertheless elevated chromosome loss. N-Ta...
ORGANISM(S): Schizosaccharomyces pombe 
2015-04-16 | E-GEOD-63350 | biostudies-arrayexpress
GenomicsMediator promotes CENP-A incorporation at fission yeast centromeres [ChIP-seq]
At Schizosaccharomyces pombe centromeres, heterochromatin formation is required for de novo incorporation of the histone H3 variant CENP-A/Cnp1, which in turn directs kinetochore assembly and ultimately chromosome segregation during mitosis. Noncoding RNAs (ncRNAs) transcribed by RNA polymerase II (...
ORGANISM(S): Schizosaccharomyces pombe 
2012-11-06 | E-GEOD-35524 | biostudies-arrayexpress
GenomicsEic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly
CENP-A chromatin the foundation for kinetochore assembly in all eukaryotes. CENP-A is incorporated outside of S phase and depends on its chaperone HJURPScm3, and Mis18 in vertebrates and fission yeast. The recruitment of Mis18 and HJURPScm3 to centromeres is cell cycle regulated. Vertebrate Mis18 a...
ORGANISM(S): Schizosaccharomyces pombe 
2014-05-19 | E-GEOD-54685 | biostudies-arrayexpress
TranscriptomicsMultiome snRNA + snATAC on MCF10-2A cells to study how high CENP-A levels promote EMT
CENP-A, the centromeric histone H3 variant, is essential for the proper segregation of human chromosomes to daughter cells. High CENP-A expression leads to the misincorporation of CENP-A in non-centromeric chromatin and can promote an epithelial-mesenchymal transition (EMT). Here, we explored how C...
ORGANISM(S): Homo sapiens 
2026-04-24 | E-MTAB-15582 | biostudies-arrayexpress
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