Genome-wide maps of WT and over-expressing CenH3/CENP-A in Human HeLa S3 cells

ABSTRACT: Centromeres are essential to ensure proper chromosome segregation in eukaryotes. Their definition relies on the presence of a centromere-specific H3 histone variant CenH3, known as CENP-A in mammals. Its overexpression in aggressive cancers raises questions concerning its effect on chromatin dynamics and contribution to tumorigenesis. We find that CenH3 overexpression in human cells leads to ectopic enrichment at sites of active histone turnover. Furthermore, in over-expressing conditions, we see the formation of a novel heterotypic particle (CenH3-H4/H3.3-H4) that occludes CTCF binding, but this occlusion has only a minor effect on gene expression. Ectopic localization and CTCF occlusion depends on the H3.3 chaperone DAXX, rather than its dedicated chaperone HJURP. This DAXX-dependent occ

ORGANISM(S): Homo sapiens

SUBMITTER: Genevieve Almouzni 

PROVIDER: E-GEOD-42951 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress