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Proteomicsrat myocardial left ventricular tissue proteins LC-MSMS
Rat left ventricular tissue LC-MSMS.Rat left ventricular tissue proteins from different groups were extracted and quantified using the BCA Protein Assay Kit (Thermo Fisher Scientific, USA). The proteins were then labeled with tandem mass tags (TMT). TMT6/10 (Pierce, USA) with different reporter ions...
ORGANISM(S): Rattus norvegicus (Rat) 
2021-07-26 | PXD024641 | Pride
Rat Heart Lc-msms 
ProteomicsHuman Plasma Proteome with Three-dimensional Strategies by Highresolution Quadrupole Orbitrap Mass Spectrometry
Five three-dimensional strategies combining HAP depletion (the 1st dimension) and protein fractionation (the 2nd dimension), followed by LC-MS/MS analysis (the 3rd dimension) were developed and compared for human plasma proteome profiling. Pros and cons of the five strategies are discussed for two i...
ORGANISM(S): Homo Sapiens 
2017-06-20 | PXD008441 |
Proteomics,MultiomicsCHPP chr 1,8,20 proteome dataset using the HCC cell lines of Hep3B and MHCC97H, instrument is AB5600, part 3 of 3
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-so...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-20 | MSV000080256 | MassIVE
Proteomics,MultiomicsCHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, instrument is Q Exactive, part 1 of 3
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-s...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-20 | MSV000080255 | MassIVE
Proteomics,MultiomicsCHPP chr 1,8,20 proteome dataset using the HCC cell lines of Hep3B and MHCC97H, instrument is Q Exactive, part 2 of 3
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-so...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-20 | MSV000080254 | MassIVE
Proteomics,MultiomicsHs_HCC_r2
CHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, part 2
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005197 | PeptideAtlas
Proteomics,MultiomicsHs_HCC_r3
CHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, part 3
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005194 | PeptideAtlas
Proteomics,MultiomicsHs_HCC_r1
CHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, part 1
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005189 | PeptideAtlas
ProteomicsThe MS data for accuracy evaluation of absolute quantification algorithms
The DDA and DIA data for accuracy evaluation of absolute quantification algorithms.
ORGANISM(S): Mus Musculus Saccharomyces Cerevisiae 
2018-05-08 | PXD009719 |
ProteomicsThe MS data for precision evaluation of absolute quantification algorithms
Human embryonic kidney (HEK) cells 293 are analyzed by two state-of-the-art MS instruments (Thermo Orbitrap Fusion and Q Exactive HF) in quadruplicate for precision evaluation.
ORGANISM(S): Homo Sapiens 
2018-05-08 | PXD009720 |
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