In order to gain insights into how PPARg regulates different facets of dendritic cell (DC) differentiation, we sought to identify PPARg regulated genes and gene networks in monocyte-derived dendritic cells using global gene expression profiling. We employed an exogenous ligand activation approach us...
Bacteria were grown in BHI until OD600 1.0 (late exponential phase) and RNA stabilized using the RNAprotectTM bacteria reagent (QIAGEN) according to the manufacturer recommendations. For each strain, RNA extraction has been carried out on three independent biological samples. Briefly, bacterial tota...
We infected wild type L. monocytogenes EGD-e (1) and its isogenic deltahlydeltaplcA (2) (lacking the ability to breach the vacuolar compartment of host cells following uptake) mutant strain to human intestinal epithelial cell line (Caco-2) with an MOI of 100 and 500 respectively. Bacterial total RNA...
5 human fecal gut samples, collected and prepared for standard MudPIT data collection from healthy volunteers, searched with the ComPIL database. x3 replicates each
