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GenomicsThe nucleosomal barrier to promoter escape by RNA Polymerase II is overcome by the chromatin remodeler Chd1 [HiSeq]
We use a metabolic labeling strategy for directly measuring nucleosome turnover to determine the role of chromatin remodeler Chd1 on chromatin dynamics in mouse embryonic fibroblasts expressing with wildtype-Chd1 and dominant negative K510R-Chd1. We find that expression of K5 10R-Chd1 decreases nucl...
ORGANISM(S): Mus musculus 
2014-04-15 | E-GEOD-52501 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of prostate cells with CHD1 knockdown
Gene expression profiling of prostate tumor cells comparing cells that have CHD1 knockdown by shRNA (RWPE-1, OPCN2) or siRNA (LNCaP) with cells treated with non-targeted control. Prostate cell lines had CHD1 knockdown via shRNA or siRNA were hybridized against cells that were targeted with non-targe...
ORGANISM(S): Homo sapiens 
2011-12-19 | E-GEOD-33531 | biostudies-arrayexpress
GenomicsCo-dependency of H2B monoubiquitination and nucleosome re-assembly on Chd1
Monoubiquitination of histone H2B on lysine 123  (H2BK123ub) is a transient histone modification considered to be essential for establishing H3K4 and H3K79 trimethylation by Set1/COMPASS and Dot1, respectively.  Many of the factors such as Rad6/Bre1, the Paf1 complex, and the Bur1/Bur2 complex were ...
ORGANISM(S): Saccharomyces cerevisiae 
2012-05-01 | E-GEOD-36334 | biostudies-arrayexpress
TranscriptomicsExpression data from Chd1-deficient mouse ES cells (E14 cell lines) and genome-wide binding of Chd1 in parental ES cells
We used microarrays to study the effect of Chd1 loss of function in mouse ES cells. We used ChIP-chip to analyze genome-wide binding of Chd1 in normal ES cells. Mouse Embryonic Stem (ES) cells were infected with a lentiviral vector (pSicoR-mCherry) for expression of a shRNA against Chd1 and GFP (as ...
ORGANISM(S): Mus musculus 
2009-08-13 | E-GEOD-16462 | biostudies-arrayexpress
ProteomicsCHD1_Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME)
Elucidate the interactome of CHD1 protein in prostate cancer
ORGANISM(S): Mus musculus (Mouse) 
2025-03-14 | PXD061854 | Pride
Chd1 rime prostate cancer 
GenomicsThe ISW1 and CHD1 ATP-dependent chromatin remodelers compete to set nucleosome spacing in vivo
We addressed the roles of three nucleosome spacing enzymes (ISW1, ISW2 and CHD1) in specifying chromatin organization in S. cerevisiae. Comparison of budding yeast nucleosome positions obtained using micrococcal nuclease digestion followed by paired-end sequencing (MNase-seq). We sequenced nucleosom...
ORGANISM(S): Saccharomyces cerevisiae 
2016-02-17 | E-GEOD-69400 | biostudies-arrayexpress
GenomicsChd1 co-occupancy with early transcription elongation factors
We report the genome-wide Chd1 co-occupancy with early transcription elongation factors ChIP-seq for Chd1 and elongating RNA polymerase II
ORGANISM(S): Saccharomyces cerevisiae BY4741 
2014-10-23 | E-GEOD-56061 | biostudies-arrayexpress
TranscriptomicsIdentification of the tumor suppressor gene at chromosome 5q21
This SuperSeries is composed of the following subset Series: GSE29227: aCGH profiling of prostate tumors GSE29228: 5q21 tiling array on prostate tumors GSE33531: Gene expression profiling of prostate cells with CHD1 knockdown Refer to individual Series
ORGANISM(S): Homo sapiens 
2011-12-19 | E-GEOD-29229 | biostudies-arrayexpress
ProteomicsNucleosome recognition and DNA distortion by the Chd1 remodeler revealed at 2.6 Å resolution
Chromatin remodelers are ATP-dependent enzymes that reorganize nucleosomes within all eukaryotic genomes. The Chd1 remodeler specializes in shifting nucleosomes into evenly spaced arrays, a defining characteristic of chromatin in gene bodies that blocks spurious transcription initiation. Linked to s...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) Xenopus laevis (African clawed frog) 
2022-02-23 | PXD025287 | Pride
Chd1 Nucleosome Xl-ms Chromatin remodeler 
ProteomicsChd1 regulates repair of promoter-proximal DNA breaks to sustain hypertranscription in embryonic stem cells
Stem and progenitor cells undergo a global elevation of nascent transcription, or hypertranscription, during key developmental transitions involving rapid cell proliferation. The chromatin remodeler Chd1 binds to RNA Pol I and II genes and is required for hypertranscription in embryonic stem (ES) ce...
ORGANISM(S): Mus musculus (Mouse) 
2021-06-14 | PXD024604 | Pride
Mouse stem cell orbitrap immunoprecipitation mass spectrometry 
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