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TranscriptomicsRequirement for flap endonuclease 1 (FEN1) to maintain genomic stability and transcriptional gene silencing in Arabidopsis
As a central component during Okazaki fragment maturation, flap endonuclease 1 (FEN1) removes the 5’ flap and maintains genomic stability. Here, FEN1 was cloned as a suppressor of transcriptional gene silencing (TGS) from a forward genetic screen. FEN1 is abundant in the root and shoot apical meri...
ORGANISM(S): Arabidopsis thaliana 
2016-05-28 | E-GEOD-79738 | biostudies-arrayexpress
ProteomicsAnalysis of phosphorylation sites of ZmRR1 phosphorylated by ZmMAPK8
To identify the phosphorylation site of ZmRR1 by ZmMPK8 in vitro, 10 µg GST-ZmRR1 and 1 µg His-ZmMPK8 purified proteins were incubated in 20 µl of protein kinase reaction buffer containing 20 mM MgCl2, 50 mM Tris-HCl pH 7.5, 1 mM DTT, and 50 µM ATP, at 30°C for 30 min. The reaction products were red...
ORGANISM(S): Zea Mays 
2021-07-16 | PXD027378 |
ProteomicsNUT1-Exo70A1 Regulates Xylem Vessel Development and Influences Water Use Efficiency in Maize
The total proteins from transgenic plants overexpressing Exo70A1 coding sequence in a translational fusion of a GFP-tag were extracted from root stele tissues using a lysis buffer composed of 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5 mM EDTA pH 8.0, 0.5% NP-40, and freshly added 1 mM PMSF, 1 mM DTT, 1...
ORGANISM(S): Zea Mays 
2026-01-20 | PXD073273 |
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