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ProteomicsExtended DNA threading through a dual-engine motor module in the activating signal co-integrator complex
Activating signal co-integrator complex (ASCC) supports diverse genome maintenance and gene expression processes. Its ASCC3 subunit is an unconventional nucleic acid helicase, harboring tandem Ski2-like NTPase/helicase cassettes crucial for ASCC functions. Presently, the molecular mechanisms underly...
ORGANISM(S): Homo sapiens (Human) 
2023-03-06 | PXD036106 | Pride
Protein-dna cross-linking 
ProteomicsStructural basis of human transcription-DNA repair coupling
Transcription-coupled DNA repair (TCR) efficiently removes bulky DNA lesions from transcribed parts of the genome and constitutes a major defence against DNA damage by UV irradiation. TCR begins when RNA polymerase II (Pol II) stalls at a DNA lesion and recruits the Cockayne syndrome protein CsB, th...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2022-02-17 | PXD025328 | Pride
Protein-protein crosslinking Transcription-dna repair Ubiquitination analysis 
ProteomicsStructural basis of TFIIH activation for nucleotide excision repair
Nucleotide excision repair (NER) is the major DNA repair pathway that removes UV-induced and bulky DNA lesions. There is currently no structure of NER intermediates, which form around the large multisubunit transcription factor IIH (TFIIH). Here we report the cryo-EM structure of an NER intermediate...
ORGANISM(S): Homo sapiens (Human) 
2019-07-02 | PXD013947 | Pride
Ner pathway Bs3 cross-linking 
ProteomicsThe previously uncharacterized YlxR (RnpM) protein modulates the activity of ribonuclease P
Even though Bacillus subtilis is one of the most studied organisms, no function has been identified for about 20% of its proteins. Among these unknown proteins are several RNA- and ribosome-binding proteins suggesting that they exert functions in cellular information processing. In this work, we hav...
ORGANISM(S): Bacillus subtilis 
2024-06-16 | PXD043900 | Pride
Bacillus subtilis Protein-rna crosslinking 
ProteomicsELOF1 anchors CRL4CSA to RNA pol II to activate transcription-coupled repair
Transcription-coupled DNA repair (TCR) removes transcription-blocking DNA lesions through the coordinated assembly of repair factors on arrested RNA polymerase II (Pol II). This process requires the site-specific ubiquitylation of Pol II by the CRL4CSA ubiquitin ligase. Pol II ubiquitylation is faci...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2023-11-24 | PXD042388 | Pride
Bs3 crosslinking Transcription-coupled dna repair 
ProteomicsAtg9 resides on a unique population of small vesicles in presynaptic nerve terminals
In neurons, autophagosomes are mainly initiated in distal axons, followed by maturation during retrograde transport. Autophagosomal growth depends on the supply of membrane lipids which requires small vesicles containing Atg9, a lipid scramblase essential for autophagocytosis. Here, we show that Atg...
ORGANISM(S): Rattus norvegicus (Rat) 
2024-01-17 | PXD041702 | Pride
Neurons Immunoisolation Atg9 
ProteomicsStructural basis for the c-di-AMP - dependent regulation of the stringent response by DarB
The bacterial second messenger c-di-AMP controls various cellular processes including potassium and osmolyte homeostasis. The c-di-AMP receptor protein DarB of Bacillus subtilis binds to the Rel protein and triggers the Rel-dependent stringent response. Here we report crystal structures of DarB in t...
ORGANISM(S): Bacillus subtilis subsp. subtilis 
2022-08-12 | PXD033346 | Pride
Cx-ms Bs3 Darb 
ProteomicsGPATCH4 regulates rRNA and snRNA 2’-O-methylation in both DHX15-dependent and DHX15-independent mannersGPATCH4 regulates rRNA and snRNA 2’-O-methylation in both DHX15-dependent and DHX15-independent manners
Regulation of RNA helicase activity, often accomplished by protein cofactors, is essential to ensure target specify within the complex cellular environment. The largest family of RNA helicase cofactors are the G-patch proteins, but the cognate RNA helicases and cellular functions of numerous human G...
ORGANISM(S): Homo sapiens (Human) 
2025-08-15 | PXD043301 | Pride
Small nucleolar rna Rna-protein crosslinking Rna modification 2’-o-methylation Ribosome Ribosome biogenesis Rna helicase 
UnknownStructural insights into lipid membrane binding by human ferlins
Not available
S-SCDT-10_1038-S44318-025-00463-8 | biostudies-other
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