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Blue Native-PAGE resolves protein complexes in their native state. When coupled with immunoblotting, it can be used to identify the presence of high molecular weight complexes at high resolution for any protein, given a suitable antibody. To identify proteins in high molecular weight complexes on a ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-12-23 | PXD003162 | Pride
Collaborative assembly of additional complete sugarcane chloroplasts from whole genome data
Palaquium complete chloroplasts
As the organelles in which photosynthesis occurs, chloroplasts are extremely susceptible to saline-alkali stress. Proteins from chloroplast were identified and quantified by isobaric tags for relative and absolute quantification (iTRAQ) strategy, and searched with both of Uniprot Liliposida database...
ORGANISM(S): Oryza sativa (Rice) 
2021-09-08 | PXD005491 | Pride
To confirm the localization of GCSC1 to chloroplast vesicle rather than in the stroma, we purified chloroplasts from WT plants and fractionated the proteins into the membrane and stroma fractions. Quantification of proteins by liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that G...
ORGANISM(S): Oryza sativa (Rice) 
2024-09-26 | PXD056230 | Pride
Sustainable production of myoglobin meat protein in plant chloroplasts
Assembly of Saccharum species chloroplasts using magnetic bead capture and MinION sequencing
Photosynthesis is the energetic basis for most life on Earth, and in plants it operates inside double-membrane-bound organelles called chloroplasts. The photosynthetic apparatus comprises numerous proteins encoded by the nuclear and organellar genomes. Maintenance of this apparatus requires the acti...
ORGANISM(S): Arabidopsis Thaliana 
2022-01-31 | PXD031468 |
Plastids contain multiple copies of the plastid chromosome, folded together with proteins and RNA into nucleoids. The degree to which components of the plastid gene expression and protein biogenesis machineries are nucleoid associated, and the factors involved in plastid DNA organization, repair, an...
ORGANISM(S): Zea mays (Maize) 
2023-09-28 | PXD045331 | Pride
Gene expression in plastids of higher plants is dependent on two different transcription machineries, a plastid-encoded bacterial-type RNA polymerase (PEP) and a nuclear-encoded phage-type RNA polymerase (NEP), which recognize distinct types of promoters. The division of labor between PEP and NEP du...
ORGANISM(S): Hordeum vulgare 
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