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ProteomicsSequential Phosphoproteomic Enrichment through Complementary Metal-Directed Immobilized Metal Ion Affinity Chromatography
We developed a tip-based sequential IMAC based on different kinds of metal ion for increasing the coverage of phosphoproteome. This approach not only can be used for singly and multiply phosphopeptides separation but also for acidic, basophilic and Pro-directed phosphopeptides separation.
ORGANISM(S): Homo Sapiens (human) 
2016-09-07 | PXD004903 | JPOST Repository
ProteomicsHs_Lung_Phospho_SequentialIMAC_tissue
Sequential Phosphoproteomic Enrichment by Complementary MD-IMAC, Cancer tissues
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005323 | PeptideAtlas
ProteomicsHs_Lung_Phospho_SequentialIMAC_cell
Sequential Phosphoproteomic Enrichment by Complementary MD-IMAC, Raji cell line
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005320 | PeptideAtlas
ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
To increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showi...
ORGANISM(S): Homo Sapiens (human) 
2016-09-08 | PXD004904 | JPOST Repository
ProteomicsMining Missing Protein Landscape in Subcellular Proteome of Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) have capacity of self-renewal and multi-lineage differentiation into derivatives of three germ layers that are of clinical importance for regeneration medicine. Despite the significant progress of hESCs study for clinical application, factors responsible for mainta...
ORGANISM(S): Homo Sapiens (human) 
2019-05-20 | PXD009840 | JPOST Repository
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_H1975
Mining Missing Membrane Proteins, Hp-RP StageTips, H1975
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005413 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_H3255
Mining Missing Membrane Proteins, Hp-RP StageTips, H3255
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005336 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_CL97
Mining Missing Membrane Proteins, Hp-RP StageTips, CL97
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005449 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_CL83
Mining Missing Membrane Proteins, Hp-RP StageTips, CL83
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005364 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_CL100
Mining Missing Membrane Proteins, Hp-RP StageTips, CL100
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005443 | PeptideAtlas
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