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TranscriptomicsTranscriptional analysis of fluorescence-activated sorted Mesp-GFP-labeled B7.5 founder trunk ventral cell lineage cells from Ciona intestinalis
The Ciona heart progenitor lineage (TVC, trunk ventral cells) is first specified by Fibroblast Growth Factor/Map Kinase (FGF/MapK) activation of the transcription factor Ets1/2 (Ets). For this analysis, B7.5 lineage cells were labeled with the Mesp-GFP reporter. In the first experiment, we targeted ...
ORGANISM(S): Ciona intestinalis 
2012-06-04 | E-GEOD-38313 | biostudies-arrayexpress
TranscriptomicsGene expression profiles of unfertilized eggs of Ciona intestinalis
The transcriptome of unfertilized eggs of Ciona intestinalis was determined with microarrays. To obtain gene expression profiles of unfertilized eggs, we took 14 biological replicates. For the first through fourth replicates, we also took a technical duplicate. Gene expression profiles of the 8-, 16...
ORGANISM(S): Ciona intestinalis 
2013-10-29 | E-GEOD-45500 | biostudies-arrayexpress
TranscriptomicsGene expression profiles of individual blastomeres of early Ciona embryos
We examined gene expression profiles of individual blastomeres of early Ciona embryos using microarrays. We examined gene expression profiles of individual blastomeres of the 8-cell, 16-cell and 32-cell embryos by dissecting with a fine glass needle. We also examined gene expression profiles of unfe...
ORGANISM(S): Ciona intestinalis 
2013-10-29 | E-GEOD-45548 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA-seq of the 16-cell stage Ciona embryo
Ciona develops according to a stereotyped or invariant lineage. This allowed us to collect precisely defined replicates of all eight blastomeres of the right half of the embryo at the 16-cell stage of Ciona. We manually dissected each embryo, collecting and identifying each cell before library prepa...
ORGANISM(S): Ciona robusta 
2020-03-06 | E-MTAB-6117 | biostudies-arrayexpress
TranscriptomicsA distinct class of small RNAs arises from pre-miRNA-proximal regions in a simple chordate
We report the comprehensive sequencing of small RNA libraries created from different developmental stages of the basal chordate, Ciona intestinalis. Surprisingly, half of the identified miRNA loci encode not two, but up to four distinct small RNAs. These additional RNAs, termed M-bM-^@M-^\moRsM-bM-...
ORGANISM(S): Drosophila melanogaster 
2009-01-18 | E-GEOD-13625 | biostudies-arrayexpress
TranscriptomicsComparative analysis of gene expression profiles between four kinds of blastomere at 8-cell stage embryos.
The establishment of body axes and specification of early embryonic cells depend on maternally supplied transcripts and/or proteins, several of which are localized at specific regions of fertilized eggs and early embryos. The ascidian is known to exhibit a mosaic mode of development, and this mode ...
ORGANISM(S): Ciona intestinalis 
2010-06-19 | E-GEOD-15637 | biostudies-arrayexpress
ProteomicsQuantitative Multi-Omics Atlas of the Model Chordate Ciona
We present a comprehensive proteome atlas of the model chordate Ciona, covering eight developmental stages and ~7k translated genes as well as a deep quantitative atlas of maternal proteins found in the Ciona egg.
ORGANISM(S): Ciona robusta 
2023-11-28 | PXD043619 | Pride
Embryogenesis Proteomics Development Ciona 
TranscriptomicsComparative analysis of gene expression profiles between the ovary treated with or without Ci-TK
Tachykinins (TKs) and their receptors have been shown to be expressed in the mammalian ovary. However, the biological roles for ovarian TKs have yet to be verified. Ci-TK and Ci-TK-R, characterized from the protochordate (ascidian), Ciona intestinalis, are prototypes of vertebrate TKs and their rece...
ORGANISM(S): Ciona intestinalis 
2010-05-22 | E-GEOD-8821 | biostudies-arrayexpress
TranscriptomicsCiona intestinalis (ascidian): control vs. TBT exposed
Transcriptional profiling of Ciona intestinalis comparing ethanol exposed asidian (control) with TBT exposed ascidian. Two conditions experiment comparing ethanol exposed asidian (control) with TBT exposed ascidian.
ORGANISM(S): Ciona intestinalis 
2010-07-20 | E-GEOD-22995 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of B7.5 lineage cells in the tunicate Ciona intestinalis to study cardiac cell migration
Eight different types of sample (three samples each) were used in this study of cardiac cell migration in the tunicate Ciona intestinalis. The B7.5 lineage cells were isolated by Fluorescent Activated Cell Sorting (FACS) at the tailbud stage, when the cardiac precursors (anterior B7.5 lineage cells)...
ORGANISM(S): Ciona intestinalis 
2008-06-01 | E-MEXP-1478 | biostudies-arrayexpress
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