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Prp4-1 and wt strains were grown at 26°C to A600 of 1.0, then an equal volume of 48°C media was added to bring the temperature to 37°C. Both strains were allowed to grow at 37°C and samples were taken at 0 (before shift), 5, 15, 30, 60, and 120 mins after shift to restrictive temperature. Keywo...
ORGANISM(S): Saccharomyces cerevisiae 
Set of experiments done on yeast deletion strains of various non-essential mRNA processing factors. Keywords = splicing
ORGANISM(S): Saccharomyces cerevisiae 
Differentiating erythroid cells execute a unique gene expression program that insures synthesis of the appropriate proteome at each stage of maturation. Standard expression microarrays provide important insight into erythroid gene expression, but cannot detect qualitative changes in transcript struc...
ORGANISM(S): Homo sapiens 
Six bacterial genomes, Geobacter metallireducens GS-15, Chromohalobacter salexigens, Vibrio breoganii 1C-10, Bacillus cereus ATCC 10987, Campylobacter jejuni subsp. jejuni 81-176 and Campylobacter jejuni NCTC 11168, all of which had previously been sequenced using other platforms were re-sequenced u...
ORGANISM(S): Geobacter metallireducens 
Splicing and expression analysis of a collection of 80 different mutations to the gene expression pathway in yeast
ORGANISM(S): Saccharomyces cerevisiae 
In addition to the differences between populations in transcriptional and translational regulation of genes, alternative pre-mRNA splicing (AS) is also likely to play an important role in regulating gene expression and generating variation in mRNA and protein isoforms. Recently, the genetic contribu...
ORGANISM(S): Homo sapiens 
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