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TranscriptomicsExpression or knock-down of ZC3H32 in Trypanosoma brucei
Bloodstream forms with 24h RNAi against ZC3H32 or procyclic forms with 24h ZC3H32 expression.
ORGANISM(S): Trypanosoma brucei 
2017-03-15 | E-MTAB-5615 | biostudies-arrayexpress
TranscriptomicsRNA-IP of TAP-tagged ZC3H32 in bloodstream-form Trypanosoma brucei
TAP RNA-IP - ZC3H32 (Tb927.10.5250)
ORGANISM(S): Trypanosoma brucei 
2017-12-07 | E-MTAB-5612 | biostudies-arrayexpress
TranscriptomicsRNA-seq to investigate effect of what shock on procyclic-form trypanosomes - ribosomal subunit
These are the ribosomal subunit fractions from the polysome gradients. investigating effect of heat shock on procyclic-form trypanosomes.
ORGANISM(S): Trypanosoma brucei brucei 
2022-01-22 | E-MTAB-11314 | biostudies-arrayexpress
TranscriptomicsWild-type Lister 427 Bloodstream-form polysome profiles with ZC3H32 knock-down
Ran from polysomes of Lister 427 bloodstream forms, wild-type and a cell line with a double knockout and inducible ZC3H32, grown without tetracycline.
ORGANISM(S): Trypanosoma brucei 
2017-11-24 | E-MTAB-4558 | biostudies-arrayexpress
GenomicsInteractions of CAF1-NOT complex components from Trypanosoma brucei
Our trypanosome yeast two-hybrid prey library was made by random shotgun genomic cloning. NOT2, NOT10, NOT11 and CAF40 were used as baits to screen the library by mating. Diploid progeny were subjected to selection, resulting in between 100 and 800 surviving colonies, from which inserts were amplifi...
ORGANISM(S): Trypanosoma brucei 
2022-01-14 | E-MTAB-4946 | biostudies-arrayexpress
GenomicsGenomic DNA T b. brucei and T. b. rhodesiense. Data for E-MTAB-3800
Genomic DNA T b. brucei and T. b. rhodesiense. These are actually paired reads but the files are unpaired
ORGANISM(S): Trypanosoma brucei 
2021-12-17 | E-MTAB-11303 | biostudies-arrayexpress
TranscriptomicsTranscriptome of Trypanosome brucei after PUF2 Knock-down - polysome fractions
Polysome fractions of RNAi PUF2 (Tb927.10.12660) 13 hours RNAi induction (TET)
ORGANISM(S): Trypanosoma brucei 
2018-07-16 | E-MTAB-5613 | biostudies-arrayexpress
TranscriptomicsPUF2 and UBP1 RNAi and pull-down in Trypanosoma brucei
PUF2 RNAi, V5-PUF2 and UBP1-myc pull-downs, all methods and protocols are described in detail in the publication
ORGANISM(S): Trypanosoma brucei 
2018-05-21 | E-MTAB-5617 | biostudies-arrayexpress
GenomicsGenomic sequencing of T. brucei cell lines resistant to AN7973
Wild type T. brucei bloodstream form were incubated with increasing concentrations of AN7973, a benzoxaborole compound developed by Anacor Pharmaceuticals Inc. against Animal African Trypanosomiasis. Cells had a tendency to lose resistance, only roughly 2x EC50 resistance was obtained. This experime...
ORGANISM(S): Trypanosoma brucei brucei 
2018-06-04 | E-MTAB-6307 | biostudies-arrayexpress
ProteomicsProteins interacting with ZC3H28 and DRBD18
ZC3H28 is a zinc-finger domain protein that is essential in bloodstream forms trypanosomes. Tethering ZC3H28 to a reporter mRNA results in an increase of the reporter mRNA levels but without a corresponding increase in protein expression levels. ZC3H28 associates with long RNAs that have low complex...
ORGANISM(S): Trypanosoma brucei 
2022-10-14 | PXD027792 | Pride
Rna-binding proteins  trypanosoma brucei  post-transcriptional regulation 
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