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TranscriptomicsRNA-Seq data: RNA-IP (TAP-ZC3H30 pull down followed by RNA-Seq) and total transcriptomics (Effect of ZC3H30 gene deletion) in procyclic form of T. brucei
The first 4 samples belong to the RNA-IP using in situ TAP tagged ZC3H30 in procyclic (insect) form of the parasite T. brucei Lister 427, 2 samples are Elu or eluate, and 2 are FL or flowthrough (unbound) sample. The other 8 samples are also from procyclic cells. 4 samples belong to DKO(ZC3H30 gene ...
ORGANISM(S): Trypanosoma brucei 
2017-12-16 | E-MTAB-6281 | biostudies-arrayexpress
TranscriptomicsRNAseq of ZC3H5 interacting mRNA
Bloodstream-form trypanosomes (Lister 427) constitutively expressing ZC3H5-TAP (Tb927.3.740) were used. The protein was pulled down with an IgG column , then the protein and bound RNA was eluted using TEV protease. RNA was then sequenced from unbound (flow-through) and bound (eluate) fractions.
ORGANISM(S): Trypanosoma brucei brucei TREU927 
2020-06-06 | E-MTAB-9084 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of procyclic Trypanosoma brucei cultured at 27oC or under heat shock stress conditions at 41oC
Procyclic trypanosomes (strain 427 lister) were grown in culture under standard conditions at 27ºC in SDM79 medium with 10% foetal bovine serume (Brun and Schnenberger, 1979), in a gazed incubator (5% CO2). Logarithmically growing procyclic cells (at about 5*10^6 cells/ml, at 27°C) were added to o...
ORGANISM(S): Trypanosoma brucei 
2008-09-10 | E-MEXP-1476 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Trypanosoma bruceii DHH1 wild type from pLEW100 for 24 hours
Induction of the expression of the DHH1 wild type from pLEW100 for 24 hours (M-1 24 hours Tetracycline). Non-induced cells were used as control.
ORGANISM(S): Trypanosoma brucei 
2010-01-01 | E-MEXP-2025 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Trypanosoma brucei DHH1 DEAD:DQAD mutant from pLEW100 for 24 hours
Induction of the expression of the DHH1 DEAD:DQAD mutant from pLEW100 for 24 hours (thus, M-1 24 hours
Tetracycline). Uninduced cells were used as control.
ORGANISM(S): Trypanosoma brucei 
2010-01-01 | E-MEXP-2026 | biostudies-arrayexpress
TranscriptomicsA domino effect in drug action: From metabolic assault towards parasite differentiation
Glycolysis is the sole free-energy source for the deadly parasite Trypanosoma brucei and is therefore a possible target pathway for anti-trypanosomal drugs. Plasma-membrane glucose transport exerts high control over trypanosome glycolysis and hence the transporter is a promising drug target. Here we...
ORGANISM(S): Trypanosoma brucei 
2010-10-18 | E-GEOD-24275 | biostudies-arrayexpress
GenomicsATD
WTCCC1 project Autoimmune Thyroid Disease (ATD) samples
EGAD00000000011 | EGA
GenomicsBC
WTCCC1 project Breast cancer (BC) samples
EGAD00000000013 | EGA
GenomicsMS
WTCCC1 project Multiple Sclerosis (MS) samples
EGAD00000000012 | EGA
GenomicsWTCCC2 Ankylosing spondylitis cases - individual
WTCCC1 project Ankylosing Spondylitis (AS) samples
EGAD00000000010 | EGA
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