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ProteomicsClick-linking: a cell-compatible protein crosslinking method based on click chemistry
We developed a novel approach to in situ crosslinking mass spectrometry (XL-MS) where the reaction is split into two sequential and orthogonal coupling events. The method involves pre-stabilization of the proteome using a fixation protocol, followed by a two-step process that begins with extensive l...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-01-28 | PXD059118 | Pride
In situ Crosslinking Proteome-wide Protein-protein interactions Interactome 
TranscriptomicsProfiling of chromatin deposition bias in RPE-1 cells using Double-click-seq
We used double-click-seq method to profile chromatin deposition bias in RPE-1 cells. Untreated wild-type, p53 knock-out and several treatments (hydroxyurea, ATR inhibitor, curaxin) and their combinations were profiled for characterizing assymetry during DNA replication.
ORGANISM(S): Homo sapiens 
2021-10-04 | E-MTAB-8624 | biostudies-arrayexpress
ProteomicsIn-situ Click Reaction Coupled with Quantitative Proteomics for Identification of Protein Targets of Catechol Estrogens
Catechol estrogens (CEs) are metabolic electrophiles that actively undergo covalent interaction with cellular proteins, influencing molecular function. There is no feasible method to identify their binders in a living system. Herein, we developed a click chemistry-based approach using ethinylestradi...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-02-28 | PXD009657 | Pride
Post translational modification Metabolites Proteomics Electrophiles Dimethyl labeling Lc/ms Catechol estrogens Oxidative stress Click chemistry Redox modification 
TranscriptomicsProfiling of chromatin deposition bias in RPE-1 cells using Double-click-seq and SCAR-seq
We used double-click-seq and SCAR-seq methods to profile chromatin deposition bias in RPE-1 cells. Untreated cells and treatments promoting G4 formation (hydroxyurea, NMM) and their combination were profiled for characterizing chromatin protein redistribution asymmetry during DNA replication. This i...
ORGANISM(S): Homo sapiens 
2025-08-31 | E-MTAB-13346 | biostudies-arrayexpress
ProteomicsClick-enabled analogues reveal pregnenolone interactomes in cancer and immune cells
Click-enabled analogues reveal pregnenolone interactomes in cancer and immune cells. TMT-6 experiment of tryptic, carbamidomethylated peptides of mouse CD8+ T-cells after protein pull-down on pregnenolone beads.
ORGANISM(S): Mus musculus (Mouse) 
2021-04-26 | PXD025574 | Pride
Pregnenolone pull-down cd8+ t-cells 
GenomicsRna_seq analysis of muscle in click and EDC treated mouse
Rna_seq analysis of muscle in click and EDC treated mouse
PRJNA1054043 | ENA
GenomicsClick chemistry enables comprehensive preclinical evaluation of targeted epigenetic therapies [Click-Seq 2]
The success of targeted therapies hinges on our ability to understand the molecular and cellular mechanism of action of these agents. Here we modify various BET bromodomain inhibitors, an exemplar novel targeted therapy, to create functionally conserved compounds that are amenable to click-chemistry...
ORGANISM(S): Homo sapiens 
2017-07-13 | GSE88749 | GEO
GenomicsClick chemistry enables comprehensive preclinical evaluation of targeted epigenetic therapies [Click-Seq 1]
The success of targeted therapies hinges on our ability to understand the molecular and cellular mechanism of action of these agents. Here we modify various BET bromodomain inhibitors, an exemplar novel targeted therapy, to create functionally conserved compounds that are amenable to click-chemistry...
ORGANISM(S): Homo sapiens 
2017-07-13 | GSE88748 | GEO
GenomicsIdentification of newly synthesized proteins in microbial communities using BONCAT and click chemistry
Identification of newly synthesized proteins in microbial communities using BONCAT and click chemistry
PRJEB70937 | ENA
GenomicsDouble-click-seq allows to track new histone deposition bias in hRPE-1 cells
Double-click-seq allows to track new histone deposition bias in hRPE-1 cells
PRJEB35915 | ENA
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