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ProteomicsTop-down Proteomics of Endogenous Membrane Proteins Enabled by A Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry Top-down Proteomics of Endogenous Membrane Proteins Enabled by A Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry
Top-down proteomics has emerged as a powerful strategy to characterize proteins in biological systems. Nevertheless, the analysis of endogenous membrane proteins is challenging due to their low solubility, low abundance, and the complexity of the membrane subproteome. Here, we report a simple, effec...
ORGANISM(S): Homo sapiens (Human) 
2020-11-30 | PXD019368 | Pride
Membrane protein Top-down proteomics Cloud point 
ProteomicsNanodiamond Solid Phase Extraction and Triton X-114 Cloud Point Separation for Robust Fractionation and Shotgun Proteomics Analysis of Human Serum Proteome
Human serum is one of the most attractive specimens in biomarker research. However, its overcomplicated properties have hindered the analysis of low-abundance proteins by conventional mass spectrometry techniques. This work proposes an innovative strategy for utilizing nanodiamonds (NDs) in combinat...
ORGANISM(S): Homo sapiens (Human) 
2022-02-14 | PXD029710 | Pride
Proteomic analysis Cloud point separation Triton x-114 Prote-ome Nanodiamond Fractionation Solid phase extraction Human serum Lc-ms/ms 
UnknownAn unsupervised semantic segmentation network for wood-leaf separation in 3D point clouds.
Not available
S-EPMC12709894 | biostudies-literature
UnknownInfluence of image analysis strategy, cooling rate, and sample volume on apparent protein cloud-point temperature determination.
Not available
S-EPMC7889528 | biostudies-literature
UnknownTop-Down Proteomics of Endogenous Membrane Proteins Enabled by Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry.
Not available
S-EPMC7968110 | biostudies-literature
UnknownFormulation of chelating agent with surfactant in cloud point extraction of methylphenol in water.
Not available
S-EPMC6083667 | biostudies-literature
UnknownDevelopment of a cloud point extraction method combined with ETAAS and ICP-MS for the preconcentration and quantification of silver as nanoparticles in saline samples.
Not available
S-EPMC12222420 | biostudies-literature
UnknownCloud point extraction of chlorophylls from spinach leaves using aqueous solutions of non-ionic surfactants.
Not available
S-EPMC6161820 | biostudies-literature
UnknownSustainable Recovery of Polyphenols and Carotenoids from Horned Melon Peel via Cloud Point Extraction.
Not available
S-EPMC11431220 | biostudies-literature
UnknownSurface-Active Ionic Liquid-Assisted Cloud Point Extraction for Pre-Concentration and Determination of Cobalt Ions in Pharmaceutical Preparations.
Not available
S-EPMC9347226 | biostudies-literature
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