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ProteomicsInducible synthetic protein degradation system for phenotype control
Synthetic control of cell factory phenotypes is needed for redirecting resources from cell growth to production. To achieve that ClpXP proteasome was introduced to yeast Saccharomyces cerevisiae under control of TetON induction. Growth essential (in synthetic defined medium) metabolic enzymes Acc1, ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-05-10 | PXD034451 | Pride
Saccharomyces cerevisiae Clpxp proteasome 
UnknownInducible Synthetic Growth Regulation Using the ClpXP Proteasome Enhances cis,cis-Muconic Acid and Glycolic Acid Yields in Saccharomyces cerevisiae.
Not available
S-EPMC10127448 | biostudies-literature
UnknownDegradation of MinD oscillator complexes by Escherichia coli ClpXP.
Not available
S-EPMC7857489 | biostudies-literature
UnknownSubstrate-translocating loops regulate mechanochemical coupling and power production in AAA+ protease ClpXP.
Not available
S-EPMC5467750 | biostudies-literature
UnknownAn enhancer sequence in the intrinsically disordered region of FtsZ promotes polymer-guided substrate processing by ClpXP protease.
Not available
S-EPMC8996474 | biostudies-literature
UnknownThe asymmetry in the mature amino-terminus of ClpP facilitates a local symmetry match in ClpAP and ClpXP complexes.
Not available
S-EPMC4377234 | biostudies-literature
ProteomicsDegradation mechanism of important regulator AtrA mediated by ClpXP in Streptomyces roseosporus
3×Flag-atrA strain was grown in YEME and 50 mL were collected after 24 h. Cells were resuspended in phosphate buffer (pH=7.4) (PBS) and sonicated. After centrifugation at 12000 rpm, the supernatant was transferred and incubated with FLAG affinity gel (Yeasen) at 4 °C. The FLAG affinity gel was washe...
ORGANISM(S): Streptomyces filamentosus NRRL 15998 
2024-05-23 | PXD037377 | Pride
Daptomycin  streptomyces roesosporus proteasome 
UnknownTopologically knotted deubiquitinases exhibit unprecedented mechanostability to withstand the proteolysis by an AAA+ protease.
Not available
S-EPMC5935755 | biostudies-literature
UnknownNew role of the disulfide stress effector YjbH in β-lactam susceptibility of Staphylococcus aureus.
Not available
S-EPMC3232775 | biostudies-literature
UnknownStructure, dynamics, and stability of the smallest and most complex 71 protein knot.
Not available
S-EPMC10840475 | biostudies-literature
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