In the experimental group, the coelomic cavity of HHStage 12-13 chick embryos was electroporated with a plasmid expressing PatchedDLoop2 (a dominant negative hedgehog pathway inhibitor) and GFP. In the control group, a plasmid expressing GFP alone was electroporated. After 14 hours, embryos were dis...
ORGANISM(S): Gallus gallus