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ProteomicsOptimization of an RNA-centric approach searching for RNA-interacting proteins in Arabidopsis thaliana
The number of methods used to study RNA-protein interactions in plants is currently relatively limited. The RNA-centric approach, starting with RNA of interest, brings several difficulties, especially when the RNA belongs to the less abundant RNAs. These methods for identification of RNA-interactin...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-22 | PXD046839 | Pride
Arabidopsis thaliana Telomerase Rna Rna-centric methods Rna-protein interactions Chirp-ms Telomerase rna Lc-ms/ms 
ProteomicsIdentification of U1 snRNA associated proteins
In this project, we isolate U1 snRNA associated proteins in Arabidopsis thaliana. We used an antisense oligonucleotide specific for the U1 snRNA and analyzed associated proteins by mass spectrometry. As a control, the same experiments were performed with U2 snRNA- and lacZ-specifc antisense oligonuc...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-12-04 | PXD045484 | Pride
U1 snrna Arabidopsis U2 snrna Comprehensive isolation by rna binding proteins (chirp) 
ProteomicsPull-down experiment of Cdkn1a mRNA and its protein binding partners in wounds and homeostatic skin of porcine skin
To elucidate the mechanism responsible for the absence of p21 (protein) in homeostatic porcine skin and its rapid generation upon injury, we performed a pull-down experiment of Cdkn1a mRNA and its protein binding partners. For this, we utilized a variant of the comprehensive identification of RNA bi...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2026-03-31 | PXD075222 | Pride
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