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Annotation of metabolites is an essential, yet problematic, aspect of mass spectrometry (MS)-based metabolomics assays. The current repertoire of definitive annotations of metabolite spectra in public MS databases is limited and suffers from lack of chemical and taxonomic diversity. Furthermore, the...
2018-03-05 | MTBLS330 | MetaboLights

INTRODUCTION: The Metabolomics Standards Initiative has recommended four categories for metabolite assignments in NMR-based metabolic profiling studies. The putatively annotated compound category is most commonly reported by metabolomics investigators. However, there is significa...

2019-09-27 | MTBLS781 | MetaboLights

This study aimed to establish and validate a robust untargeted lipidomics workflow for the analysis of human serum using reversed-phase liquid chromatography coupled to trapped ion mobility spectrometry time-of-flight mass spectrometry (RP-LC-TIMS-TOF MS). Commercial pooled human serum was used a...

2026-05-26 | MTBLS14002 | MetaboLights
This study presents a mathematically rigorous approach that integrates peptide MS-signal and peptide-measurement agreement into an estimation of the true protein ratio and the associated confidence.We call our method BACIQ (Bayesian Approach to Confidence Intervals for protein Quantitation – pronoun...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2019-09-30 | PXD012285 | Pride
The Photo-Activatable Ribonucleoside-enhanced CrossLinking and ImmunoPrecipitation (PAR-CLIP) method was recently developed for global identification of RNAs interacting with proteins. The strength of this versatile method results from induction of specific T to C transitions at sites of interaction...
ORGANISM(S): Homo sapiens 
High-confidence Coding and Noncoding Transcriptome Maps
Optimal settings of mass spectrometry open search strategy for higher confidence
The Project for High-Confidence Coding and Noncoding Transcriptome Maps
a- Self –Self Hybridisation were used to set confidence 99% interval using RNA from non-tethered cell lines that is both labelled in with cy3 cy5 (theoretically identical cDNA populations) to compared technical errors associated with such experiments Keywords: comparative hybridization to access exp...
ORGANISM(S): Homo sapiens 
2008-03-04 | GSE10697 | GEO
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