To generate novel genetic markers, we performed RNA sequencing of 10 accessions of Ae. tauschii. Transcripts were deduced from de novo assembly of short reads for each accession.
Plant material consisted of synthetic hexaploid wheat germplasm into the ‘Opata’ background (‘Altar 84/ Aegilops squarrosa (TAUS)//Opata’) . Plants were grown at a density of 9-11 individuals per 20cm x 10cm (diameter x height) plastic pot containing 1500g well-rinsed Turface MVP® medium (Profile...
