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ProteomicsMETTL21B is a novel human lysine methyltransferase of translation elongation factor 1A: discovery by CRISPR/Cas9 knock out
Lysine methylation is widespread on human proteins, however the enzymes that catalyse its addition remain largely unknown. This limits our capacity to study the function and regulation of this modification. Here we report that human METTL21B is a protein methyltransferase, which methylates lysine 16...
ORGANISM(S): Homo sapiens (Human) 
2017-07-03 | PXD005497 | Pride
Methyltransferase Cas9 Knockout Crispr Methylation 
TranscriptomicsscRNA-seq CRISPR screening of human posterior brain organoids
We used a pooled, multiplexed CRISPR/Cas9 gene knock-out (KO) experiment with single-cell transcriptomic readout to perturb and validate selected TF regulomes . We designed gRNAs and generated a pooled lentiviral library targeting 12 TFs with specific midbrain or hindbrain expression or high regulat...
ORGANISM(S): Homo sapiens 
2026-03-17 | E-MTAB-15826 | biostudies-arrayexpress
MetabolomicsFSCN1 as a new druggable target in adrenocortical carcinoma

Adrenocortical carcinoma (ACC) is a rare endocrine malignancy with a high risk of relapse and metastatisation. The actin-bundling protein fascin (FSCN1) is overexpressed in aggressive ACC and represents a reliable prognostic indicator. FSCN1 has been shown to synergize with the Rho/Rac GEF VAV2 i...

2023-03-20 | MTBLS6402 | MetaboLights
GenomicsRNAseq in immortalized human podocyte cell line following PRDM15 CRISPR knock-out
RNAseq in immortalized human podocyte cell line following PRDM15 CRISPR knock-out
PRJNA671311 | ENA
ProteomicsEgg proteome in vtg1 and vtg3 knocked-out zebrafish females
CRISPR/Cas9 knock-out of the vtg1 and vtg3 genes was performed in zebrafish separately. This project involves proteomics screening for validation of the absence of targeted proteins in vtg1 and vtg3 and their variants in knocked out female zebrafish in eggs and investigating possible changes within ...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2021-09-09 | PXD021302 | Pride
Knock-out Vitellogenins Eggs Zebrafish 
GenomicsTranscriptome network analysis of Smug1 knock-out cells by using CRISPR/Cas9 system
Transcriptome network analysis of Smug1 knock-out cells by using CRISPR/Cas9 system
PRJNA606327 | ENA
GenomicsUSP7 CRISPR knock-out in T-ALL cells [Molt4_CRISPR_RNA-seq]
USP7 CRISPR knock-out in T-ALL cells [Molt4_CRISPR_RNA-seq]
PRJNA624872 | ENA
GenomicsTranscriptomic changes after CRISPR/Cas9-mediated knock-out of lncRNA LINC00607 in HUVEC [RNA-seq]
Transcriptomic changes after CRISPR/Cas9-mediated knock-out of lncRNA LINC00607 in HUVEC [RNA-seq]
PRJNA821879 | ENA
UnknownEfficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing.
Not available
S-EPMC6722079 | biostudies-literature
TranscriptomicsTranscriptome of iPSC-derived Neural Cells with Heterozygous Knockout in CHD8
CHD8, encoding Chromodomain helicase DNA binding protein 8, is a top autism spectrum disorders (ASDs) risk gene. To better understanding the molecular links between CHD8 functions and ASD, we have applied the CRISPR/Cas9 technology to knockout one copy of CHD8 in induced pluripotent stem cells (iPSC...
ORGANISM(S): Homo sapiens 
2015-11-05 | E-GEOD-71594 | biostudies-arrayexpress
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