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ProteomicsValidating the use of CRISPR interference for the modulation of glycosylation in Burkholderia thailandensis
To determine whether CRISPR interference can be used to modulate glycosylation in Burkholderia thailandensis
ORGANISM(S): Burkholderia thailandensis E264 
2023-03-20 | PXD036623 | Pride
Glycosylation Burkholderia thailandensis Crispr interference Crispri 
ProteomicsValidating the use of CRISPR interference for the modulation of glycosylation in Burkholderia cenocepacia
To determine whether CRISPR interference can be used to modulation glycosylation in Burkholderia cenocepacia
ORGANISM(S): Burkholderia cenocepacia K56-2Valvano 
2023-03-20 | PXD036564 | Pride
Glycosylation Burkholderia cenocepacia Crispr interference Crispri 
ProteomicsValidating the use of CRISPR interference for the modulation of glycosylation in Burkholderia multivorans
To determine whether CRISPR interference can be used to modulation glycosylation in Burkholderia multivorans
ORGANISM(S): Burkholderia multivorans ATCC 17616 
2023-03-20 | PXD036619 | Pride
Glycosylation Crispr interference Burkholderia multivorans Crispri 
ProteomicsValidating the use of CRISPR interference for the modulation of glycosylation in Burkholderia diffusa
To determine whether CRISPR interference can be used to silence the expression of pglL in Burkholderia diffusa and therefore modulate glycosylation
ORGANISM(S): Burkholderia diffusa 
2023-03-20 | PXD036585 | Pride
Glycosylation Burkholderia diffusa Crispr interference Crispri 
ProteomicsAnalysing the effects of glycosylation knockdown via CRISPR interference in Burkholderia diffusa
To determine the affect of CRISPR interference-mediated silencing of glycosylation in Burkholderia diffusa harbouring a pglL (WL85_RS02125)-targeted guide, and a nontarget guide using Data Independent Acquisition
ORGANISM(S): Burkholderia diffusa 
2023-03-20 | PXD037556 | Pride
Glycosylation Burkholderia diffusa Dia Crispr interference Crispri 
ProteomicsValidating the use of CRISPR interference for the modulation of glycosylation in Burkholderia cenocepacia - induction levels
To determine a suitable level of rhamnose required for the effective silencing of pglL and glycosylation using CRISPR interference in Burkholderia cenocepacia
ORGANISM(S): Burkholderia cenocepacia K56-2Valvano 
2023-03-20 | PXD036568 | Pride
Glycosylation Burkholderia cenocepacia Crispr interference Crispri 
ProteomicsStructural variation of types IV-A1- and IV-A3-mediated CRISPR interference
CRISPR-Cas mediated DNA-interference typically relies on sequence-specific binding and nucleolytic degradation of foreign genetic material. Type IV-A CRISPR-Cas systems diverge from this general mechanism, using a nuclease-independent interference pathway to suppress gene expression for gene regulat...
ORGANISM(S): Pseudomonas oleovorans 
2024-10-08 | PXD056399 | Pride
Cryo-em Type iv crispr-cas 
ProteomicsAnalysing the effects of glycosylation knockdown via CRISPR interference in Burkholderia thailandensis
To determine the effect of CRISPR interference-mediated silencing of glycosylation in Burkholderia thailandensis harbouring a pglL-targeted guide, and a nontarget guide using Data Independent Acquisition
ORGANISM(S): Burkholderia thailandensis E264 
2023-03-20 | PXD037595 | Pride
Glycosylation Burkholderia thailandensis Dia Burkholderia pseudomallei complex Crispr interference Crispri 
Proteomicsii. Analysing the effects of glycosylation knockdown via CRISPR interference in Burkholderia multivorans
To determine the effect of CRISPR interference-mediated silencing of glycosylation in Burkholderia multivorans harbouring a pglL-targeted guide, and a nontarget guide using Data Independent Acquisition
ORGANISM(S): Burkholderia multivorans ATCC 17616 
2023-03-20 | PXD037585 | Pride
Glycosylation Burkholderia multivorans Crispr interference Burkholderia cepacia complex Crispri Dia 
GenomicsIV CRISPR-Cas system displays PAM-dependent interference to vectors
IV CRISPR-Cas system displays PAM-dependent interference to vectors without showing nuclease activity to the target
PRJEB48544 | ENA
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