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Bacterial transcription is a tightly regulated process critical for gene expression, adaptation and cell survival. CarD is an essential transcription factor in mycobacteria involved in regulation of gene expression. Here, we searched for CarD interaction partners in the model organism Mycobacterium ...
ORGANISM(S): Mycobacterium smegmatis 
2025-12-22 | PXD058166 | Pride
ChIP-seq identification of CrsL (MSMEG_5890) binding sites in Mycobacterium smegmatis
CarD is an essential transcription factor for Mycobacterium smegmatis. We identified a new interaction partner of CarD and named it CrsL (MSMEG_5890). To address the genome-wide effect of CrsL, we generated knockdown strains for both carD (MSMEG_6077) and crsL (MSMEG_5890) genes in Mycobacterium sme...
ORGANISM(S): Mycolicibacterium smegmatis 
CsrL (MSMEG_5890) associates with CarD and RNA polymerase in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strain with CrsL (LK3051), where an additional copy of CrsL under anhydrotetracycline inducible promoter was stably inserted into M. smegmatis genome. CrsL-FLAG was expressed in e...
ORGANISM(S): Mycolicibacterium smegmatis MC2 155 
RNA-seq of Mycobacterium smegmatis mc2 155 negative control strain (NC), carD (MSMEG_6077) and crsL (MSMEG_5890) knockdown strains, from exponential and stationary phase cells.
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