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Age-associated B cells (ABCs) accumulate in systemic autoimmunity, and contributes to pathogenesis. ABCs are characterized by high expression of CD11c and T-bet. As a transcription factor, T-bet cannot be labeled in live cells with antibodies, so we constructed a reporter mouse strain in which fluor...
ORGANISM(S): Mus musculus 
We performed the cleavage under targets and tagmentation (Cut & tag) assay followed by sequencing enriched DNA fragments to reveal the direct downstream targets of Pbx1. Firstly, we overexpressed Pbx1b with Pbx1b-IRES-GFP retrovirus in murine peripheral B cells to ensure the yields of DNA fragments....
ORGANISM(S): Mus musculus 

A total of 445 and 247 differently expressed metabolites (DEMs) were identified in the hepatopancreas and muscle. In addition, 11 KEGG pathways, which mainly include lysine biosynthesis, lysine degradation, aminoacyl-tRNA biosynthesis and purine metabolism, were sign...

2023-02-28 | MTBLS6663 | MetaboLights
We aim to see what T4 phage and/or E. coli proteins interact with our DRT1 enzyme. We have conducted a pulldown assay using N-terminally MBP-tagged DRT1, both for WT and RT-inactive mutant.
ORGANISM(S): Escherichia Coli 
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