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TranscriptomicsIdentification of HP1 protein binding sites in Drosophila Kc cells using DamID
Heterochromatin protein HP1 is thought to play key role in chromatin structure and gene regulation. We performed a genome-wide mapping of HP1 target genes in the non-polytenic Drosophila Kc cells by using DamID. This approach is based on the ability of a chromatin protein fused to Escherichia coli D...
ORGANISM(S): Drosophila melanogaster 
2007-07-05 | E-MEXP-864 | biostudies-arrayexpress
TranscriptomicsIdentification of SUUR protein binding sites in Drosophila Kc cells using DamID
Salivary gland polytene chromosomes of Drosophila melanogaster have a reproducible set of intercalary heterochromatin sites, characterized by late DNA replication, underreplicated DNA, breaks and frequent ectopic contacts. The SuUR mutation has been shown to suppress underreplication, and wild-type ...
ORGANISM(S): Drosophila melanogaster 
2007-07-04 | E-MEXP-863 | biostudies-arrayexpress
TranscriptomicsDamID (LmnB1/Dam) Log2 ratios of C57Bl/6 fibroblasts and RAG2-/- pro-B cells.
Comparison of DamID profiles and LAD patterning across cell types reveals regions of variable LADs LmnB1 interactions at the nuclear periphery of C57Bl/6 pro-B cells and fibroblasts were identified by DamID in duplicate for both pro-B and fibroblasts (4 samples total). For each sample, Dam-LmnB1/Dam...
ORGANISM(S): Mus musculus 
2014-12-16 | E-GEOD-56990 | biostudies-arrayexpress
TranscriptomicsThe Hox gene Abd-B controls stem cell niche function in the Drosophila testis
Stem cells reside in a specialized microenvironment, called niche, which provides essential signals controlling stem cell behavior. Proper niche architecture is a key for normal stem cell function, yet only few upstream regulators are known. Here we report that the Hox transcription factor Abd-B, ac...
ORGANISM(S): Drosophila melanogaster 
2013-12-30 | E-GEOD-53709 | biostudies-arrayexpress
GenomicsDam(ID)-profiling in B cells
Both chromatin access, and binding of AID in B cells are of major scientific interest. Therefore DamID was setup in B cells. AID specific binding could not be identified. In contrast to binding, DAM profiling provide a upmost useful tool in defining chromatin access in vivo.
ORGANISM(S): Homo sapiens 
2016-05-12 | GSE81342 | GEO
GenomicsDNA accessibility profile of three C. elegans tissues during development
We show there is minimal genome-wide chromatin rearrangements (as measured by DNA accessibility) during tissue differentiation in C. elegans transgenic expression of E. coli DAM (DNA Adenine Methyltransferase) from tissue-specific promoters followed by mapping of methylated sites using a procedure t...
ORGANISM(S): Caenorhabditis elegans 
2011-04-16 | E-GEOD-23042 | biostudies-arrayexpress
TranscriptomicsGenome-wide profiling of PRC1 and PRC2 Polycomb chromatin binding in Drosophila
Polycomb group (PcG) proteins maintain transcriptional repression of developmentally important genes and have been implicated in cell proliferation and stem-cell self-renewal. We used a genome-wide approach to map binding patterns of PcG proteins (Pc, esc and Sce) in Drosophila Kc cells. We found th...
ORGANISM(S): Drosophila melanogaster 
2010-06-11 | E-GEOD-4564 | biostudies-arrayexpress
TranscriptomicsDamID of Dam::LMN-1 and Dam::EMR-1 in Caenorhabditis elegans adult worms
Laminopathies are caused by mutations in components of the nuclear envelope (NE). While most NE components are widely expressed, laminopathies affect only a subset of tissues. However, the understanding of the molecular mechanisms that explain this phenomenon is still elusive. Here we have performed...
ORGANISM(S): Caenorhabditis elegans 
2013-12-02 | E-GEOD-44188 | biostudies-arrayexpress
GenomicsHomo sapiens
Dam(ID)-profiling in B cells
PRJNA321322 | ENA
TranscriptomicsB-type lamins differentially associate with specific genes during differentiation without directly affecting their expression
B-type lamins, the major structural component of the nuclear lamina, is thought to play a repressive role in the expression of many of its bound genes whose silencing are known to be critical for cell differentiation during early development. Using global mapping of lamin-B-chromatin interaction, w...
ORGANISM(S): Mus musculus 
2011-10-31 | E-GEOD-24532 | biostudies-arrayexpress
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