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UnknownA High-Sensitivity Low-Nanoflow LC-MS Configuration for High-Throughput Sample-Limited Proteomics.
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S-EPMC10753523 | biostudies-literature
ProteomicsRepurposed 3D Printer Allows Economical and Programmable Fraction Collection for Proteomics of Nanogram Scale Samples
In this work we describe the construction and application of a repurposed 3D-printer as a fraction collector. We utilise a nanoLC to ensure minimal volumes and surfaces although any LC can be coupled. The setup operates as a high-pH fractionation system capable of effectively working with nanogram s...
ORGANISM(S): Homo sapiens (Human) 
2024-07-12 | PXD051148 | Pride
Dda dia wwa lc-ms/ms Phosphoproteome Proteomics Two-dimensional peptide separation Rna-binding protein 
ProteomicsWide-Window Acquisition Improves Proteome Coverage and Measurement Throughput for Label-Free Single-Cell Proteomics
The sensitivity of single-cell proteomics (SCP) has increased dramatically in recent years due to advances in experimental design, sample preparation, separations and mass spectrometry instrumentation. However, further increasing the sensitivity of SCP methods and instrumentation will enable the stu...
ORGANISM(S): Homo sapiens (Human) 
2023-07-11 | PXD037527 | Pride
Label-free Proteome Single-cell proteomics Wide-window acquisition 
ProteomicsBenchmarking CHIMERYS using Wide Window Acquisition and Classical Narrow Isolation Window Data Dependent Acquisition
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-for...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2024-02-13 | PXD045500 | Pride
E. coli Wide window acquisition Yeast Direct injection Micropillar array chromatography Vanquish neo Hela Orbitrap exploris 480 Single cells Trap-and-elute 
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