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UnknownFirst Dimension Trap-and-Elute Combined with Second Dimension Reversed-Phase Liquid Chromatography Separation Using a Two-Dimensional-Liquid Chromatography-Tandem Mass Spectrometry System for Sensitive Quantification of Human Insulin and Six Insulin Analogs in Plasma: Improved Chromatographic Resolution and Stability Testing.
Not available
S-EPMC11811267 | biostudies-literature
Proteomics15 cm Aurora Gen3 column benchmarking on an Exploris 480
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-for...
ORGANISM(S): Homo sapiens (Human) 
2024-02-13 | PXD045457 | Pride
Vanquish neo Orbitrap exploris 480 Trap-and-elute 
ProteomicsInteraction of SARS-CoV-2 nucleocapsid protein and human RNA helicases DDX1 and DDX3X modulates their activities on double-stranded RNA
The proteomic analysis described in this study was performed on A549 cells transfected with an empty vector and with SARS-CoV-2 N protein by means of a label free shotgun platform, consisting of an Eksigent nanoLC-Ultra 2D System configured in trap-elute mode coupled to a LTQ-OrbitrapXL mass spectro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-12-31 | MSV000090979 | MassIVE
UnknownA High-Sensitivity Low-Nanoflow LC-MS Configuration for High-Throughput Sample-Limited Proteomics.
Not available
S-EPMC10753523 | biostudies-literature
ProteomicsBenchmarking CHIMERYS using Wide Window Acquisition and Classical Narrow Isolation Window Data Dependent Acquisition
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-for...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2024-02-13 | PXD045500 | Pride
E. coli Wide window acquisition Yeast Direct injection Micropillar array chromatography Vanquish neo Hela Orbitrap exploris 480 Single cells Trap-and-elute 
ProteomicsBenchmarking of 5.5 cm, 50 cm and 110 cm micropillar array columns as compared to packed bed columns
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-for...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2024-02-13 | PXD039576 | Pride
E. coli Yeast Direct injection Micropillar array chromatography Vanquish neo Hela Orbitrap exploris 480 Trap-and-elute 
UnknownEx vivo instability of glycated albumin: A role for autoxidative glycation.
Not available
S-EPMC5568244 | biostudies-literature
UnknownA Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-robust Proteomics.
Not available
S-EPMC6210218 | biostudies-literature
ProteomicsA novel LC system embeds analytes in pre-formed gradients for rapid, ultra-robust proteomics
To further integrate mass spectrometry (MS)-based proteomics into biomedical research and especially into clinical settings, high throughput and robustness are essential requirements. They are largely met in high-flow rate chromatographic systems for small molecules but these are not sufficiently se...
ORGANISM(S): Homo sapiens (Human) 
2018-08-16 | PXD010393 | Pride
Clinic Plasma Liquid chromatography 
UnknownEffects of the LC mobile phase in vacuum differential mobility spectrometry-mass spectrometry for the selective analysis of antidepressant drugs in human plasma.
Not available
S-EPMC9482904 | biostudies-literature
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