Sort   by:  
 Page size 
ProteomicsEvaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation
This work comprehensively compared 50 mM of ammonium acetate (pH 6), Tris-HCl (pH 8), ABC and TEAB as in-solution trypsin digestion buffers. Both iTRAQ quantification and label-free results indicate that ammonium acetate (pH 6) is more suitable than other buffers for studying endogenous deamidation ...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-02-28 | PXD001650 | Pride
Rat Kidney Artificial deamidation 
ProteomicsMilking it? Assessing the degradation of ancient milk proteomes
The origins, prevalence and nature of dairying have been long debated by archaeologists. Within the last decade, new advances in high-resolution mass spectrometry have allowed for the direct detection of milk proteins from archaeological remains, including ceramics, dental calculus, and preserved da...
ORGANISM(S): Homo sapiens (Human) 
2021-08-05 | PXD022368 | Pride
Milk Authentication Palaeoproteomics Deamidation 
ProteomicsOxidative damage signatures in human bone collagen from Pompeii and Herculaneum.
In search of markers for protein aging, we used an extraordinary test case of human bones from the excavations sites of Pompei and Herculaneum (79 AD), analyzed by a shotgun proteomic approach using LC-MS/MS. Human bones from Scalandrone Bay (II sec AD, another volcanic area, yet not related to Vesu...
ORGANISM(S): Homo sapiens (Human) 
2021-07-10 | PXD020462 | Pride
Paleoproteomics Human bones Pompeii Ages Deamidation (nq) Vesuvius 79 ad herculaneum Shotgun proteomics 
ProteomicsProteomic profile of Cephalosporin-Resistant Neisseria gonorrhoeae Isolates by low pH nano-HPLC-MS/MS
The experiment was performed using three N. gonorrhoeae clinical isolates and N. gonorrhoeae strain ATCC 49226 was used as a reference strain. Tandem mass spectra were processed by PEAKS Studio version 8.5 (Bioinformatics Solutions Inc., Waterloo, Canada). PEAKS DB was set up to search the database ...
ORGANISM(S): Neisseria gonorrhoeae 
2019-10-22 | PXD015296 | Pride
Low ph nano-hplc-ms/ms  cephalosporin-resistant neisseria gonorrhoeae 
ProteomicsRedox proteomics in NO-mediated protection on hypoxic cardiomyocyte
We developed a mass spectrometry (MS)-based quantitative approach using a set of novel iodoacetyl-based cysteine-reactive isobaric tags (iodoTMT) endowed with unique irreversible Cys-reactivities to differentially quantify the multiple redox-modified forms of a Cys site in the original cellular cont...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-06-27 | PXD000274 | Pride
S-nitrosylation Mass spectrometry Hypoxia Redox cysteine modifications Redox-proteomics 
ProteomicsIndentification of B7H3 WT/NQ N-Glycosites
Most patients with triple negative breast cancer (TNBC) fail to respond to anti-PD1/PDL1 immunotherapy, indicating the necessity to explore immune checkpoint targets. B7H3 is a highly glycosylated protein. However, the mechanisms of B7H3 glycosylation regulation and whether the sugar moiety contribu...
ORGANISM(S): Homo sapiens (Human) 
2021-03-15 | PXD024672 | Pride
Glycosites Breast cancer B7h3 
ProteomicsNam Lot enamel proteome: part of a multi-proxy approach to exploring Homo sapiens arrival, environments and adaptations in Southeast Asia
Here we analyse two enamel protein extractions from a single Late Pleistocene dental fragment from Nam Lot (Laos). The proteome is analyzed for potential phylogenetic information, from which we concude the specimen represents an individual belonging to the genus Pongo, and characterized in terms of ...
ORGANISM(S): Pongo sp. 
2021-11-30 | PXD027426 | Pride
Palaeproteomics Human evolution Enamel Pleistocene Lc-ms/ms 
ProteomicsQuantitative Proteomics of miR-148a in gastric cancer cells
A quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins in AGS cancer cells. For proteomic analysis, cells were treated with miR-148a mimic (Pre-miR-148a) or miR-148a negative control (miR-CTL) and the downs...
ORGANISM(S): Homo sapiens (Human) 
2013-07-24 | PXD000190 | Pride
Mir-148a Quantitative proteome Itraq 
ProteomicsDNA damage-induced alternative splicing of p53
Samples were reduced in loading buffer and separated by SDS-PAGE for 3-4 min followed by in-gel digestion with trypsin. Digests were analyzed by LC-MS/MS using a Waters nanoACQUITY interfaced to a Thermo Fusion Lumos MS. The LC separation 90 min gradient of 5-30% MeCN in a trapping configurations w...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-12-30 | MSV000086651 | MassIVE
ProteomicsRapid High-Throughput Antibody Analysis Using Microwave-Assisted Digestion
Monoclonal antibodies (mAbs) are a major class of biotherapeutic proteins that have been extensively developed over the past decade, resulting in improved standards of care for multiple diseases. Regulatory agencies recommend a Quality by Design (QbD) approach for manufacturing therapeutic proteins,...
ORGANISM(S): Homo sapiens (Human) 
2026-01-26 | PXD067893 | Pride
Data independent acquisition Post translational modifications Antibodies Data dependent acquisition Microwave-assisted digestion 
Sort   by:  
 Page size