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UnknownA simple heat-based alternative method for deparaffinization of histological sections significantly improves acid-fast staining results for Mycobacteria in tissue.
Not available
S-EPMC9971263 | biostudies-literature
ProteomicsA non-hazardous deparaffinization protocol enables quantita-tive proteomics of core needle biopsy-sized formalin-fixed and paraffin embedded (FFPE) tissue specimens.
Most human tumor tissues that are obtained for pathology and diagnostic purposes are forma-lin-fixed and paraffin embedded (FFPE). To perform quantitative proteomics of FFPE samples, paraffin has to be removed and formalin-induced crosslinks have to be reversed prior to prote-olytic digestion. A c...
ORGANISM(S): Homo sapiens (Human) 
2022-05-19 | PXD031946 | Pride
Clinical proteomics  tumor tissues  ffpe  quantitative proteomics  core needle biopsy  cancer research  molecular pathology  breast ductal carcinoma  in-situ cancer 
UnknownA Non-Hazardous Deparaffinization Protocol Enables Quantitative Proteomics of Core Needle Biopsy-Sized Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens.
Not available
S-EPMC9031572 | biostudies-literature
ProteomicsTemperature, Oil and Pressure (TOP) extraction: optimal sample preparation for proteomic analysis of formalin fixed paraffin embedded tissue
Formalin-fixed paraffin embedded (FFPE) is the clinical gold standard of tissue preservation globally. We present a novel method for optimal extraction of proteins from FFPE tissue samples. The method combines non-toxic, proteomic inert mineral oil for deparaffinization, followed by extraction using...
ORGANISM(S): Homo sapiens (Human) 
2024-02-12 | PXD002526 | Pride
Protein extraction Proteomics Ffpe Quantification 
ProteomicsProtein extraction method improvement for FFPE kidney tissue samples
The vast majority of tissue biopsies from patients in hospital environments are included in paraffin (FFPE- formalin-fixed and paraffin-embedded) for long-term storage. This fixation process produces a modification in the proteins called "crosslinks", which improves protein stability, necessary for ...
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD023891 | Pride
Deparaffinization  proteins extraction  formalin-fixed paraffin-embedded (ffpe) samples  proteomic approaches  lc-maldi-ms  lc-ms/ms  methylene bridges 
TranscriptomicsOptimizing microarray gene expression profiling workflow for formalin-fixed paraffin-embedded tissues
The use of nucleic acids from formalin-fixed paraffin-embedded (FFPE) tissues for high-throughput molecular techniques, such as microarray gene expression profiling has become widespread in molecular research area. However, working with FFPE tissues is challenging because of degradation, cross-linki...
ORGANISM(S): Homo sapiens 
2015-10-10 | GSE73883 | GEO
ProteomicsSimple Workflow for Integrated and Fast Tissue-preparation for microgram-level FF and FFPE Tissues Proteomics Analysis
We present Simple Workflow for Integrated and Fast Tissue-preparation (SWIFT) workflows, enabling complete processing of fresh-frozen (FF) and formalin-fixed, paraffin-embedded (FFPE) tissues in one- or two-step formats, while maintaining deep proteome coverage with good repeatability. For FF tissue...
ORGANISM(S): Homo Sapiens Mus Musculus 
PXD077679 |
UnknownComparing RNA extraction protocols from formalin-fixed paraffin-embedded microcore samples.
Not available
S-EPMC12685168 | biostudies-literature
UnknownProof of Concept Study: Comparison of Semi-Automated RNA Isolation Methods from Archived Formalin-Fixed, Paraffin-Embedded Tissues with Clinical Routine RNA Isolation Methods.
Not available
S-EPMC11678837 | biostudies-literature
UnknownEffect of Different Proteinase K Digest Protocols and Deparaffinization Methods on Yield and Integrity of DNA Extracted From Formalin-fixed, Paraffin-embedded Tissue.
Not available
S-EPMC7045302 | biostudies-literature
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