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MetabolomicsMetabolically-incorporated deuterium in myelin localized by neutron diffraction and identified by mass spectrometry
Myelin is a natural and dynamic multilamellar membrane structure that continues to be of significant biological and neurological interest, especially with respect to its biosynthesis and assembly during its normal formation and maintenance, and pathological breakdown. To explore the usefulness of ne...
2024-09-27 | MTBLS4717 | MetaboLights
ProteomicsThe Deuterium Calculator HDX-MS Data Analysis
Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is a powerful protein footprinting technique to study protein dynamics and binding; however, HDX-MS data analysis is often challenging and time consuming. Moreover, the HDX community is expanding to investigate multi-protein and highly complex p...
ORGANISM(S): Escherichia coli 
2023-01-30 | PXD036813 | Pride
Hydrogen-deuterium exchange mass spectrometry Subzero temperature uplc Hdx data analysis software Hdx-ms 
Genomics
High-deuterium sequencing
PRJNA1125856 | ENA
ProteomicsSlight deuterium enrichment in water acts as an antioxidant: is deuterium a cell growth regulator?
Small admixtures in water, e.g. of metal ions, often act as cell growth regulators. Here we report that enrichment of deuterium content in water, normally found at 8 mM concentration, two-three folds increases cell proliferation and lowers the oxidative stress level as well. Acting as an anti-oxidan...
ORGANISM(S): Homo sapiens (Human) 
2020-08-10 | PXD018216 | Pride
Cell growth regulator Antioxidant Deuterium-enriched water Oxidantion-reduction balance 
TranscriptomicsTranscription profiling of Arabidopsis after deuterium oxide treatment
2H2O has a long history as a protein or amino acid labeling techinique, and such labeling systems have proven effective for many different types of studies. A disadvantage of a 2H2O labeling system is that plant growth is inhibited as the percentage of deuterium in the medium increases. However the ...
ORGANISM(S): Arabidopsis thaliana 
2009-09-25 | E-GEOD-18153 | biostudies-arrayexpress
ProteomicsON-TISSUE DIRECT MONITORING OF GLOBAL HYDROGEN/DEUTERIUM EXCHANGE BY MALDI MASS SPECTROMETRY: TDXMS
Hydrogen/deuterium exchange mass spectrometric methods for protein structural analysis are conventionally performed in solution. We present Tissue Deuterium Exchange Mass Spectrometry (TDXMS), a method to directly monitor deuterium uptake on tissue, as a means to better approximate the deuterium exc...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-08-15 | PXD004630 | Pride
Tissue Mass spectrometry In situ analysis H/d exchange 
TranscriptomicsEffect of deuterium oxide on Arabidopsis gene expression
2H2O has a long history as a protein or amino acid labeling techinique, and such labeling systems have proven effective for many different types of studies. A disadvantage of a 2H2O labeling system is that plant growth is inhibited as the percentage of deuterium in the medium increases. However the ...
ORGANISM(S): Arabidopsis thaliana 
2009-09-18 | GSE18153 | GEO
ProteomicsDnaK interaction and folding of firefly luciferase studied by hydrogen/deuterium exchange – mass spectrometry
The ATP-dependent chaperones of the Hsp70 class (DnaK in E. coli) function in protein folding in cooperation with J proteins and nucleotide exchange factors (DnaJ and GrpE in E. coli, respectively). Hsp70 prevents protein aggregation, increasing the folding yield, but whether it also enhances the ra...
ORGANISM(S): Escherichia coli 
2020-01-15 | PXD016509 | Pride
Deuterium exchange Chaperone Firefly luciferase Dnak Folding 
ProteomicsRevealing the dynamic allosteric changes required for formation of the cysteine synthase complex by hydrogen-deuterium exchange mass spectrometry
CysE and CysK, the last two enzymes of the cysteine biosynthetic pathway, engage in a bienzyme complex, cysteine synthase (CS), with yet incompletely characterized three-dimensional structure and regulatory function. Being absent in mammals, the two enzymes and their complex are attractive targets f...
ORGANISM(S): Escherichia coli 
2021-06-07 | PXD025300 | Pride
Cysteine biosynthesis Allosteric regulation E. coli Synapt g2-si Bienzyme complex Hydrogen deuterium exchange (hdx-ms) Protein-protein interactions Cysteine synthase 
ProteomicsComplementary Backbone and Side-chain Analysis of Drug – Protein Interactions: Combining Hydrogen/Deuterium Exchange and Protein Oxidative Footprinting to Discriminate the Binding of Small Molecule Therapeutics
Characterizing and distinguishing protein-ligand interactions is crucial for understanding cellular metabolism and guiding drug discovery and development. We employ hydrogen/deuterium exchange mass spectrometry (HDX-MS) and a new Fenton chemistry-based approach to protein oxidative mass spectrometry...
ORGANISM(S): Homo sapiens (Human) 
2026-03-27 | PXD050663 | Pride
Hdx Ox-ms Bcl2 Binding 
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