Control (non-targeting) and Dia2-targeting siRNA were transfected into murine-derived mesenchymal stem cells and mRNA-Seq analysis was performed two days after transfection.
Disassembly of the DNA helicase known as CMG (Cdc45-Mcm-GINS) is the key regulated step during DNA replication termination in eukaryotes. In budding yeast, CMG disassembly is initiated by the ubiquitin ligase SCF-Dia2, which ubiquitylates the Mcm7 subunit of CMG, leading to recruitment of the Cdc48...
