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ProteomicsMining Missing Membrane Proteins by High-pH Reverse Phase StageTip Fractionation and Validation by Multiple Reaction Monitoring Mass Spectrometry
Several significant efforts have been made in the past decade to map all human proteome. However, more than 3000 proteins are grouped as missing having no evidence of translation. Membrane protein annotation of the current missing proteins list showed 34%to be missing membrane proteins, which are ch...
ORGANISM(S): Homo sapiens (Human) 
2015-07-27 | PXD002224 | Pride
High-ph reverse phase Stagetip Missing proteins Lung cancer 
ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
To increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showi...
ORGANISM(S): Homo Sapiens (human) 
2016-09-08 | PXD004904 | JPOST Repository
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_CL83
Mining Missing Membrane Proteins, Hp-RP StageTips, CL83
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005364 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_A549
Mining Missing Membrane Proteins, Hp-RP StageTips, A549
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005358 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_H3255
Mining Missing Membrane Proteins, Hp-RP StageTips, H3255
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005336 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_PC9
Mining Missing Membrane Proteins, Hp-RP StageTips, PC9
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005379 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_CL152
Mining Missing Membrane Proteins, Hp-RP StageTips, CL152
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005416 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_CL141
Mining Missing Membrane Proteins, Hp-RP StageTips, CL141
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005374 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_PC9IR
Mining Missing Membrane Proteins, Hp-RP StageTips, PC9IR
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005414 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_H1975
Mining Missing Membrane Proteins, Hp-RP StageTips, H1975
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005413 | PeptideAtlas
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