Sort   by:  
 Page size 
ProteomicsSART3 promotes homologous recombination repair by stimulating DNA–RNA hybrids removal and DNA end resection
DNA–RNA hybrids triggered by double-strand breaks (DSBs) are crucial intermediates during DSB repair, and their timely resolution requires numbers of RNA helicases, including DEAD box 1 (DDX1). However, how these helicases are recruited to DSB-induced hybrids in time remains largely unclear. Here, w...
ORGANISM(S): Homo sapiens (Human) 
2025-01-30 | PXD053595 | Pride
Sart3 Homologous recombination repair Dna–rna hybrids 
ProteomicsHuman SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility
Super killer (SKI) complex is a well-known cytoplasmic 3′ to 5′ mRNA decay complex that functions with the exosome to degrade excessive and aberrant mRNAs. Recently, SKIV2L, the 3′ to 5′ RNA helicase of the human SKI (hSKI) complex has been implicated with the extraction of mRNA at stalled ribosomes...
ORGANISM(S): Homo sapiens (Human) 
2024-10-03 | PXD046955 | Pride
Skiv2l Genome instability Dna-rna hybrids Dna damage Telomere 
ProteomicsImmunoprecipitation of RNA-DNA hybrid interacting proteins in Trypanosoma brucei reveals conserved and novel activities, including in host immune evasion by antigenic variation
RNA-DNA hybrids are widespread epigenetic features of genomes that provide a growing range of activities in transcription, chromatin and DNA replication and repair. Understanding of these diverse functions has been advanced by characterising the proteins that interact with the hybrids, with all such...
ORGANISM(S): Trypanosoma brucei 
2024-01-26 | PXD042146 | Pride
R loops  dna-rna interactors 
TranscriptomicsGenome-wide profiling of DNA methylation in two Arabidopsis ecotypes and their reciprocal hybrids - small RNA-seq
Heterosis is a fundamental biological phenomenon characterized by the superior performance of a hybrid over its parents in many traits, but the underlying molecular basis remains elusive. To investigate whether DNA methylation plays a role in heterosis, we compared at single base-pair resolution th...
ORGANISM(S): Arabidopsis thaliana 
2011-12-22 | E-GEOD-34656 | biostudies-arrayexpress
TranscriptomicsGenome-wide profiling of DNA methylation in two Arabidopsis ecotypes and their reciprocal hybrids - mRNA-seq
Heterosis is a fundamental biological phenomenon characterized by the superior performance of a hybrid over its parents in many traits, but the underlying molecular basis remains elusive. To investigate whether DNA methylation plays a role in heterosis, we compared at single base-pair resolution th...
ORGANISM(S): Arabidopsis thaliana 
2011-12-22 | E-GEOD-34655 | biostudies-arrayexpress
GenomicsGenome-wide distribution of RNA-DNA hybrids identifies RNase H targets in tRNA genes, retrotransposons and mitochondria
During transcription the nascent RNA can invade the DNA template, forming extended RNA-DNA duplexes (R-loops). Here we employ ChIP-seq in strains expressing or lacking RNase H to map targets of RNase H activity throughout budding yeast genome. In wild-type strains, R-loops were readily detected over...
ORGANISM(S): Saccharomyces cerevisiae 
2014-11-03 | E-GEOD-53420 | biostudies-arrayexpress
TranscriptomicsMassive sequencing of hybrid DNA obtained by DNA:RNA immunoprecipitation (DRIP) in extracellular microvesicles (MV) produced by HeLa cells (using S9.6 antibody)
Hybrid (Bound) and non-hybrid (Unbound) DNA from Hela EV was isolated using DRIP procedure as follows. Between 1-5μg of nucleic acid DNA from EVs (DNase0 treated) was incubated overnight with 1μg of anti-DNA-RNA Hybrid (S9.6) antibody (KeraFast) under rotation at 4°C in 500μl of binding buffer (10mM...
ORGANISM(S): Homo sapiens 
2023-03-30 | E-MTAB-9634 | biostudies-arrayexpress
ProteomicsRNA-DNA hybrids prevent resection at dysfunctional telomeres
At critically short telomeres TERRA RNA-DNA hybrids become stabilized and drive homology-directed repair (HDR) to delay replicative senescence. However, even at long- and intermediate-length telomeres, not subject to HDR, transient TERRA RNA-DNA hybrids form, suggestive of additional roles. Here, we...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-01-18 | PXD034986 | Pride
Lc-ms/ms 
GenomicsGenome-wide profiling of DNA methylation in two Arabidopsis ecotypes and their reciprocal hybrids - Bisulfite-seq
Heterosis is a fundamental biological phenomenon characterized by the superior performance of a hybrid over its parents in many traits, but the underlying molecular basis remains elusive. To investigate whether DNA methylation plays a role in heterosis, we compared at single base-pair resolution th...
ORGANISM(S): Arabidopsis thaliana 
2011-12-22 | E-GEOD-34657 | biostudies-arrayexpress
GenomicsTranscriptional repression accounts for RNA:DNA hybrids accumulation at DNA Double Strand breaks
Transcriptional repression accounts for RNA:DNA hybrids accumulation at DNA Double Strand breaks
PRJEB64562 | ENA
Sort   by:  
 Page size