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ProteomicsEscherichia coli can adapt its protein translocation machinery for enhanced periplasmic recombinant protein production
Recently, we engineered a tunable rhamnose promoter-based setup for the production of recombinant proteins in E. coli. This setup enabled us to show that being able to precisely set the production rate of a secretory recombinant protein is critical to enhance protein production yields in the peripla...
ORGANISM(S): Escherichia coli 
2020-03-05 | PXD013168 | Pride
E.coli Recombinant protein production Protein secretion 
ProteomicsStructural and Functional Analysis of ASFV pI73R Reveals GNB1 Binding and Host Gene Modulation
African swine fever virus (ASFV) causes a highly fatal disease in domestic pigs, resulting in substantial economic losses to the global swine industry. Vaccine development continues to be hindered by limited characterization of viral proteins and their functional redundancies. In this study, we empl...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2026-01-05 | PXD066360 | Pride
Gnb1 Asfv Virus-host interaction African swine fever virus Protein-protein interaction Pi73r Gene expression 
ProteomicsDynamics of the Escherichia coli proteome in response to the deletion of the Tat pathway
In Escherichia coli, the Twin-arginine (Tat) secretion system is one of the main routes of the protein export to the periplasm. The Tat pathway secretes a set of proteins with important physiological functions. In our study, we investigated the influence of the deactivation of the Tat pathway on the...
ORGANISM(S): Escherichia coli 
2019-02-18 | PXD008803 | Pride
Escherichia coli Stress responses Tat pathway 
ProteomicsAnalysis of Escherichia coli CyDisCo proteome changes in response to the expression of scFv and the misfolded scFv
E. coli CyDisCo strain enables a high yield secretion of disulfide bond-containing proteins to the periplasm via Twin-arginine (Tat) pathway. Introducing two exogenous oxidases: the yeast sulfydryl oxidase (Erv1p) and human protein disulfide isomerase (PDI), the CyDisCo strain changes the cytoplasm ...
ORGANISM(S): Escherichia coli 
2019-02-18 | PXD010078 | Pride
Tat system Stress response Escherichia coli Proteome 
ProteomicsAfrican swine fever virus protein CP204L is involved in endosomal trafficking and interacts with the VPS39 subunit of the HOPS complex
African swine fever virus (ASFV) is a large DNA virus causing a highly contagious disease in domestic and wild boar, for which no treatment is available. ASFV vaccine development is hindered by large gaps in knowledge considering virus protein function and virus-host interaction. This study shows th...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2023-01-23 | PXD035695 | Pride
Cp204l Hops complex Asfv Vps39 Virus-host ppi 
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