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ProteomicsInfection by wMel Wolbachia alters female post-mating behaviors and physiology in the dengue vector mosquito Aedes aegypti
Globally invasive Aedes aegypti mosquitoes disseminate numerous arboviruses that impact human health. One promising method to control Ae. aegypti populations is transinfection with the intracellular bacterium Wolbachia pipientis, a symbiont that naturally infects ~40-52% of insects but is normally a...
ORGANISM(S): Aedes aegypti aegypti 
2023-10-24 | PXD043965 | Pride
Female reproductive tract Seminal fluid Sperm Reproduction 
TranscriptomicsSpermatozoal mRNA is reproducibly detected in purified D. melanogaster sperm.
It is now well established that mature mammalian spermatozoa carry a population of mRNA molecules, at least some of which are transferred to the oocyte at fertilisation. However, the function of the sperm transcriptome remains largely unclear. To shed light on the evolutionary conservation of this...
ORGANISM(S): Drosophila melanogaster 
2012-04-23 | E-GEOD-33947 | biostudies-arrayexpress
ProteomicsProteomic diversification of spermatostyles among six species of whirligig beetles
Seminal fluid protein (SFP) composition is complex and commonly assumed to be rapidly divergent due to functional interactions with both sperm and the female reproductive tract (FRT), both of which evolve rapidly. In addition to sperm, seminal fluid may contain structures, such as mating plugs and s...
ORGANISM(S): Dineutus hornii Dineutus discolor Orectochilus villosus Dineutus nigrior Dineutus ciliatus Dineutus assimilis 
2024-08-09 | PXD048928 | Pride
Reproduction Fertility Sexual selection Evolution Coleoptera 
ProteomicsFemale mating status influences protein composition of mature Drosophila oocytes
Oocyte composition can directly influence offspring fitness, particularly in oviparous species such as most insects, where it is the primary form of parental investment. Oocyte production is also energetically costly, dependent on female condition and responsive to external cues. We investigated whe...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-02-24 | PXD022142 | Pride
Lc-msms Parental investment Tmt Maternal effects Oocyte Drosophila Oogenesis 
ProteomicsThe life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts: heavy-female isotopic labeling to estimate abundances of female-derived sperm protein
Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used sex-specific isotopic labeling to estimate the relative abundance of ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-03-14 | PXD027806 | Pride
Lc-msms Female reproductive tract Fertility Sperm Silac Drosophila melanogaster 
ProteomicsThe life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts
Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used whole-cell quantitative proteomics to track the protein composition o...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-03-14 | PXD027807 | Pride
Lc-msms Female reproductive tract Fertility Sperm Drosophila melanogaster 
ProteomicsThe life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts: heavy-male isotopic labeling to identify female-derived sperm proteins
Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used sex-specific isotopic labeling to identify female-derived proteins th...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-03-11 | PXD027802 | Pride
Lc-msms Female reproductive tract Fertility Sperm Silac Drosophila melanogaster 
ProteomicsExpansion of the Drosophila melanogaster sperm proteome
Given the continued advances in mass spectrometry technology and methods for database searching since the previous characterization of the Drosophila melanogaster sperm proteome, a new proteomic analysis of sperm samples was conducted to expand the size and coverage of the sperm proteome. This datas...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-03-14 | PXD027808 | Pride
Lc-msms Fertility Sperm Seminal fluid proteins Drosophila melanogaster 
ProteomicsOvarian fluid proteome variation associates with sperm swimming speed in an externally fertilizing fish
Ovarian fluid was collected from 6 adult female Chinook salmon during their annual spawning run. Two replicates were analyzed for each ovarian fluid sample fraction using 80 and 100 minute gradients, respectively. Protein abundances were estimated using iTRAQ.
ORGANISM(S): Oncorhynchus tshawytscha 
2021-04-17 | PXD014551 | Pride
Oncorhynchus tshawytscha Chinook salmon Ovarian fluid Coelomic fluid Itraq 
ProteomicsDynamic variation in ejaculate expenditure and seminal fluid proteome over a mating sequence: the signature of female novelty and male social status in red junglefowl
Seminal fluid sample were collected from dominant and subdominant males who were re peatedly mated to first female followed by a mating to the novel second female. The goal in to monitor seminal fluid investment and composition changed dynamically over the successive matings. Our results indicate th...
ORGANISM(S): Gallus gallus (Chicken) 
2019-11-14 | PXD010214 | Pride
Coolidge asdf Seminal fluid Red jungle fowl Gallus 
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